Glentis Alexandros, Blanch-Mercader Carles, Balasubramaniam Lakshmi, Saw Thuan Beng, d'Alessandro Joseph, Janel Sebastien, Douanier Audrey, Delaval Benedicte, Lafont Frank, Lim Chwee Teck, Delacour Delphine, Prost Jacques, Xi Wang, Ladoux Benoit
Université Paris Cité, CNRS, Institut Jacques Monod, F-75013 Paris, France.
Laboratoire Physico Chimie Curie, UMR 168, Institut Curie, PSL Research University, CNRS, Sorbonne Université, 75005 Paris, France.
Sci Adv. 2022 Sep 16;8(37):eabn5406. doi: 10.1126/sciadv.abn5406. Epub 2022 Sep 14.
Three-dimensional collective epithelial rotation around a given axis represents a coordinated cellular movement driving tissue morphogenesis and transformation. Questions regarding these behaviors and their relationship with substrate curvatures are intimately linked to spontaneous active matter processes and to vital morphogenetic and embryonic processes. Here, using interdisciplinary approaches, we study the dynamics of epithelial layers lining different cylindrical surfaces. We observe large-scale, persistent, and circumferential rotation in both concavely and convexly curved cylindrical tissues. While epithelia of inverse curvature show an orthogonal switch in actomyosin network orientation and opposite apicobasal polarities, their rotational movements emerge and vary similarly within a common curvature window. We further reveal that this persisting rotation requires stable cell-cell adhesion and Rac-1-dependent cell polarity. Using an active polar gel model, we unveil the different relationships of collective cell polarity and actin alignment with curvatures, which lead to coordinated rotational behavior despite the inverted curvature and cytoskeleton order.
围绕给定轴的三维集体上皮旋转代表一种驱动组织形态发生和转变的协调细胞运动。关于这些行为及其与底物曲率关系的问题与自发活性物质过程以及重要的形态发生和胚胎过程密切相关。在这里，我们采用跨学科方法研究内衬不同圆柱形表面的上皮层的动力学。我们观察到在凹面和凸面弯曲的圆柱形组织中都存在大规模、持续且圆周方向的旋转。虽然曲率相反的上皮在肌动球蛋白网络方向和相反的顶-基极性上表现出正交切换，但它们的旋转运动在共同的曲率窗口内以相似的方式出现和变化。我们进一步揭示，这种持续的旋转需要稳定的细胞间粘附和Rac-1依赖性细胞极性。使用活性极性凝胶模型，我们揭示了集体细胞极性和肌动蛋白排列与曲率的不同关系，这导致尽管曲率和细胞骨架顺序相反，但仍能产生协调的旋转行为。
