36Cl⁻离子与大鼠小脑膜的特异性结合：γ-氨基丁酸（GABA）和S-100蛋白的作用

Specific binding of 36Cl- ions to rat cerebellar membranes: effect of GABA and S-100 protein.

作者信息

Cupello A, Hydén H

出版信息

Int J Neurosci. 1987 May;34(1-2):49-54. doi: 10.3109/00207458708985939.

DOI:10.3109/00207458708985939

Abstract

36Cl- ions display specific binding to rat cerebellum membranes. Although this binding at a 136Cl-1 of 2 X 10(-7) M is only 18% of total binding, it shows several interesting characteristics. It is higher in a GABA receptor rich region such as the cerebellum than in the cerebral cortex. It is higher in synaptic than in total membranes of the cerebral cortex. 36Cl- binding to cerebellar membranes in inhibited by 10(-4) M picrotoxin and by 10(-4) M GABA, the GABA effect being antagonized by bicuculline. All these characteristics appear to point out that 36Cl- specifically binds to GABAA receptor associated Cl- channels in their closed state. The brain specific antigen S-100 also is able to inhibit 36Cl- binding to rat cerebellar membranes.

摘要

36Cl⁻离子对大鼠小脑膜表现出特异性结合。尽管在2×10⁻⁷M的³⁶Cl⁻浓度下这种结合仅占总结合量的18%，但它呈现出几个有趣的特性。在富含GABA受体的区域如小脑中，其结合量高于大脑皮层。在大脑皮层中，突触膜上的结合量高于总膜上的结合量。10⁻⁴M的印防己毒素和10⁻⁴M的GABA可抑制³⁶Cl⁻与小脑膜的结合，GABA的这种作用可被荷包牡丹碱拮抗。所有这些特性似乎都表明³⁶Cl⁻特异性结合处于关闭状态的GABAA受体相关Cl⁻通道。脑特异性抗原S - 100也能够抑制³⁶Cl⁻与大鼠小脑膜的结合。