Cupello A, Rapallino M V, Hydén H
Centro di Neurofisiologia Cerebrale, C.N.R. Genova, Italy.
Int J Neurosci. 1990 Oct;54(3-4):253-8. doi: 10.3109/00207459008986641.
The rate of accumulation of 36Cl- ions into rabbit cerebral cortex microsacs has been studied under various conditions. Such a parameter is increased by 164% by 10(-4) M GABA, the effect being reversed in the presence of 10(-4) M bicuculline and 10(-4) M picrotoxin. Incorporation of the endogenous antigen S-100, by a preincubation of the fraction with the protein in the presence of 2.5 mM Ca++, brings about a 56% stimulation of 36Cl- influx rate. This effect does not seem to be additive with that of GABA. The S-100 effect is reversed by picrotoxin but not by bicuculline. The overall pattern of data suggests an action of the antigen directly at the GABAA receptor complex and in particular at the GABA activated Cl- channel.
在不同条件下研究了36Cl-离子进入兔大脑皮层微囊的积累速率。10(-4) M的γ-氨基丁酸（GABA）可使该参数增加164%，在存在10(-4) M荷包牡丹碱和10(-4) M印防己毒素的情况下，该效应会逆转。通过在2.5 mM钙离子存在下将该组分与蛋白质进行预温育，掺入内源性抗原S-100可使36Cl-流入速率刺激56%。该效应似乎与GABA的效应不可叠加。印防己毒素可逆转S-100的效应，但荷包牡丹碱不能。数据的总体模式表明该抗原直接作用于GABAA受体复合物，特别是作用于GABA激活的氯离子通道。
