Yang Tianshu, Yang Jinsong, Tang Kai, Zhi Wenbo, Chen Rong, Tan Haisheng
College of Food Science and Engineering, Hainan University, Haikou, China.
College of Materials Science and Engineering, Hainan University, Haikou, China.
Front Microbiol. 2022 Aug 30;13:974925. doi: 10.3389/fmicb.2022.974925. eCollection 2022.
In this study, lactic acid bacteria strains (HCS-01, HCS-05, HCS-07, HCW-08, and HCW-09) derived from the gastrointestinal tract of Hainan black goat were evaluated for their antioxidant capacity , and the lactic acid bacteria with strong antioxidant capacity were screened for application to improve the aerobic stability of total mixed ration (TMR). The results showed that all the tested lactic acid bacteria had a certain tolerance to hydrogen peroxide. By comprehensively comparing the scavenging abilities of fermentation supernatants, whole cell bacterial suspensions and cell contents of five lactic acid bacteria strains to 2,2-diphenyl-1-picrylhydrazine (DPPH), hydroxyl radicals and superoxide anions, and their antioxidant enzyme activity, it was found that HCS-05 and HCW-08 have the strongest comprehensive antioxidant capacity, and their scavenging capacity for various free radicals has reached more than 60%. Using strains HCS-05, HCW-08 and laboratory-preserved HDX1 fermented TMR, the fermentation quality and aerobic stability of the feed after 60 days of fermentation were significantly higher than those of the blank treatment group. The effect of mixed strains HCS-05 and HCS-08 for TMR fermentation was the best ( < 0.05). At the same time, the fermentation effect of HDX1 on TMR was significantly lower than that of the selected lactic acid bacteria from the gastrointestinal tract of Hainan black goats ( < 0.05). The results show that the test strain can significantly improve the aerobic stability of the fermented feeds.
在本研究中，对源自海南黑山羊胃肠道的乳酸菌菌株（HCS - 01、HCS - 05、HCS - 07、HCW - 08和HCW - 09）的抗氧化能力进行了评估，并筛选出具有强抗氧化能力的乳酸菌用于提高全混合日粮（TMR）的有氧稳定性。结果表明，所有测试的乳酸菌对过氧化氢均有一定耐受性。通过综合比较5株乳酸菌菌株的发酵上清液、全细胞菌悬液和细胞内容物对2,2 - 二苯基 - 1 - 苦基肼（DPPH）、羟基自由基和超氧阴离子的清除能力及其抗氧化酶活性，发现HCS - 05和HCW - 08具有最强的综合抗氧化能力，它们对各种自由基的清除能力均达到60%以上。使用菌株HCS - 05、HCW - 08和实验室保存的HDX1对TMR进行发酵，发酵60天后饲料的发酵品质和有氧稳定性显著高于空白处理组。混合菌株HCS - 05和HCS - 08对TMR的发酵效果最佳（<0.05）。同时，HDX1对TMR的发酵效果显著低于从海南黑山羊胃肠道筛选出的乳酸菌（<0.05）。结果表明，受试菌株可显著提高发酵饲料的有氧稳定性。
