Gomes Ana Luiza Mendonça, Bueno Antônio Vinicius Iank, Osmari Milene Puntel, Machado Juliana, Nussio Luiz Gustavo, Jobim Clóves Cabreira, Daniel João Luiz Pratti
Department of Animal Science, State University of Maringá, Maringá, Brazil.
Department of Animal Science and Rural Development, Federal University of Santa Catarina, Florianópolis, Brazil.
Front Microbiol. 2021 May 10;12:643879. doi: 10.3389/fmicb.2021.643879. eCollection 2021.
Our objective was to determine the effects of two strains of obligate heterofermentative bacteria, alone or in combination, on the fermentation profile, gas production kinetics, chemical composition, and aerobic stability of sugarcane silage. A plot of sugarcane was manually harvested, mechanically chopped and treated with: distilled water (5 mL kg; Control), CNCM I-4785 [3 × 10 colony-forming units (cfu) g; LH], NCIMB 40788 (3 × 10 cfu g; LB), and LH+LB (1.5 × 10 cfu g of each strain). Treated forages were packed into 1.96-L gas-tight silos (0.40 porosity) and stored at 25 ± 1.5°C for 70 days (4 replicates per treatment). All heterolactic inoculants were effective to increase acetic acid concentration and inhibit yeast metabolism, as treated silages had lower formation of ethanol, ethyl esters and gas during fermentation. Lower fungal development spared soluble carbohydrates, consequently resulting in silages with higher digestibility. Nevertheless, was the most effective strain to extend the aerobic stability of sugarcane silage (based on both temperature and pH rise). The use of alone or in combination with , applied at 3 × 10 cfu g, is a feasible strategy to inhibit yeast metabolism and increase the nutritional quality of sugarcane silage.
我们的目标是确定两种专性异型发酵细菌菌株单独或组合使用时，对甘蔗青贮饲料的发酵特性、产气动力学、化学成分及有氧稳定性的影响。从一块甘蔗地人工收割甘蔗，机械切碎后进行如下处理：蒸馏水（5毫升/千克；对照）、法国国家微生物保藏中心（CNCM）I-4785[3×10菌落形成单位（cfu）/克；LH]、英国国家工业、食品和海洋细菌保藏中心（NCIMB）40788（3×10 cfu/克；LB），以及LH+LB（每种菌株1.5×10 cfu/克）。将处理后的草料装入1.96升气密青贮容器（孔隙率0.40），并在25±1.5°C下储存70天（每个处理4次重复）。所有异型乳酸接种剂均能有效提高乙酸浓度并抑制酵母代谢，因为处理后的青贮饲料在发酵过程中乙醇、乙酯和气体的生成量较低。真菌生长减少使可溶性碳水化合物得以保留，从而使青贮饲料的消化率更高。然而，LH是延长甘蔗青贮饲料有氧稳定性最有效的菌株（基于温度和pH值升高）。以3×10 cfu/克的用量单独使用LH或与LB组合使用，是抑制酵母代谢并提高甘蔗青贮饲料营养品质的可行策略。
