抗生素抗性质粒R100的转移起始位点序列。
oriT sequence of the antibiotic resistance plasmid R100.
作者信息
McIntire S A, Dempsey W B
出版信息
J Bacteriol. 1987 Aug;169(8):3829-32. doi: 10.1128/jb.169.8.3829-3832.1987.
Abstract
We present the nucleotide sequence of the oriT region from plasmid R100. Comparison to other IncF plasmids revealed homology around the proposed nick sites as well as conservation of inverted repeated sequences in the nonhomologous region. Three areas showed strong homology (eight of nine nucleotides) to the consensus sequence for binding of integration host factor, suggesting a role for this DNA-binding protein in nicking at oriT.
摘要
我们展示了质粒R100 oriT区域的核苷酸序列。与其他IncF质粒的比较揭示了在拟议的切口位点周围的同源性以及非同源区域中反向重复序列的保守性。三个区域与整合宿主因子结合的共有序列显示出高度同源性（九个核苷酸中有八个），表明这种DNA结合蛋白在oriT处的切口形成中发挥作用。
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