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用于提高新型碱性蛋白酶BSP-1产量的基因工程 。 （原文句子不完整，翻译可能不太能完全表意）

Genetic engineering for enhanced production of a novel alkaline protease BSP-1 in .

作者信息

Jiang Cong, Ye Changwen, Liu Yongfeng, Huang Kuo, Jiang Xuedeng, Zou Dian, Li Lu, Han Wenyuan, Wei Xuetuan

机构信息

State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.

Zhengzhou Tobacco Research Institute of China National Tobacco Corporation, Zhengzhou, China.

出版信息

Front Bioeng Biotechnol. 2022 Aug 30;10:977215. doi: 10.3389/fbioe.2022.977215. eCollection 2022.

DOI:10.3389/fbioe.2022.977215
PMID:36110310
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9468883/
Abstract

Alkaline protease has been widely applied in food, medicine, environmental protection and other industrial fields. However, the current activity and yield of alkaline protease cannot meet the demand. Therefore, it is important to identify new alkaline proteases with high activity. In this study, we cloned a potential alkaline protease gene from a strain isolated in our laboratory BSP-1 shows the highest sequence similarity to subtilisin NAT (S51909) from natto Then, we expressed BSP-1 in BAX-9 and analyzed the protein expression level under a collection of promoters. The results show that the P43 promoter resulted in the highest transcription level, protein level and enzyme activity. Finally, we obtained a maximum activity of 524.12 U/mL using the P43 promoter after fermentation medium optimization. In conclusion, this study identified an alkaline protease gene from and provided a new method for high-efficiency alkaline protease expression in .

摘要

碱性蛋白酶已广泛应用于食品、医药、环境保护等工业领域。然而，目前碱性蛋白酶的活性和产量无法满足需求。因此，鉴定具有高活性的新型碱性蛋白酶具有重要意义。在本研究中，我们从本实验室分离的一株菌株中克隆了一个潜在的碱性蛋白酶基因，该基因与纳豆枯草杆菌蛋白酶NAT（S51909）的序列相似性最高。然后，我们在BAX-9中表达BSP-1，并分析了一系列启动子下的蛋白质表达水平。结果表明，P43启动子导致最高的转录水平、蛋白质水平和酶活性。最后，通过优化发酵培养基，使用P43启动子发酵后，我们获得了524.12 U/mL的最大活性。总之，本研究鉴定了一个来自[具体来源未提及]的碱性蛋白酶基因，并为[具体宿主未提及]中高效表达碱性蛋白酶提供了一种新方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8930/9468883/f9a1862ee7c1/fbioe-10-977215-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8930/9468883/9fae0eb36efa/fbioe-10-977215-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8930/9468883/4b887470bdde/fbioe-10-977215-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8930/9468883/a56b17358287/fbioe-10-977215-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8930/9468883/36cbc38e76e6/fbioe-10-977215-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8930/9468883/c2fe9b052929/fbioe-10-977215-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8930/9468883/3a7d223fa30a/fbioe-10-977215-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8930/9468883/031a879d157f/fbioe-10-977215-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8930/9468883/f9a1862ee7c1/fbioe-10-977215-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8930/9468883/9fae0eb36efa/fbioe-10-977215-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8930/9468883/4b887470bdde/fbioe-10-977215-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8930/9468883/a56b17358287/fbioe-10-977215-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8930/9468883/36cbc38e76e6/fbioe-10-977215-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8930/9468883/c2fe9b052929/fbioe-10-977215-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8930/9468883/3a7d223fa30a/fbioe-10-977215-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8930/9468883/031a879d157f/fbioe-10-977215-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8930/9468883/f9a1862ee7c1/fbioe-10-977215-g008.jpg

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