More Prashant, Agarwal Parinita, Agarwal Pradeep K
Plant Omics Division, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific & Industrial Research (CSIR), Gijubhai Badheka Marg, Bhavnagar, Gujarat 364 002 India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002 India.
3 Biotech. 2022 Oct;12(10):275. doi: 10.1007/s13205-022-03306-z. Epub 2022 Sep 12.
Jatropha, a popular biodiesel crop, suffers severe losses due to (JLCuGV) infection in Gujarat (India). Metabolite profiling can help to understand the plant's innate immune response to geminivirus infection. Our study aims to compare metabolic profiles of an infected and healthy plant to unravel the changes in biochemical pathways on geminivirus infection in Jatropha. Gas chromatography-mass spectrometry (GC-MS) analysis was performed in healthy and infected tissue of Jatropha field plants which were identified to be infected with geminivirus. GC-MS analysis revealed that the metabolites like sugars, polyols, carboxylic acids, fatty acids, polyphenols, and amino acids were regulated on JLCuGV infection. The sugars (glucose, sucrose, and fructose) increased, while carboxylic acids (malic acid, citric acid and quinic acid) and polyols (galactinol, butanetriol, triethylene glycol, myo-inositol, erythritol) decreased remarkably in infected Jatropha tissue. All these metabolic variations indicated that sugar metabolism and tricarboxylic acid (TCA) cycle pathways are regulated as a defense response and a disease development response to geminivirus infection in Jatropha.
麻风树是一种广受欢迎的生物柴油作物，在古吉拉特邦（印度）因感染麻风树曲叶古病毒（JLCuGV）而遭受严重损失。代谢物谱分析有助于了解植物对双生病毒感染的固有免疫反应。我们的研究旨在比较受感染植物和健康植物的代谢谱，以揭示麻风树感染双生病毒后生化途径的变化。对田间被鉴定感染双生病毒的麻风树健康组织和受感染组织进行了气相色谱 - 质谱联用（GC - MS）分析。GC - MS分析表明，在感染JLCuGV后，糖类、多元醇、羧酸、脂肪酸、多酚和氨基酸等代谢物受到调控。在受感染的麻风树组织中，糖类（葡萄糖、蔗糖和果糖）增加，而羧酸（苹果酸、柠檬酸和奎尼酸）和多元醇（棉子糖、丁三醇、三甘醇、肌醇、赤藓醇）显著减少。所有这些代谢变化表明，糖代谢和三羧酸（TCA）循环途径作为对麻风树双生病毒感染的防御反应和疾病发展反应受到调控。
