Molecular characterization of adenosine monophosphate deaminase 1 and its regulatory mechanism for inosine monophosphate formation in triploid crucian carp.

Inosine monophosphate (IMP) is the main flavoring substance in aquatic animal, and adenosine monophosphate deaminase1 () gene is a key gene in IMP formation. At present, the research on the mechanism of regulating IMP formation in aquatic animal is still blank. In this study, in order to study the mechanism of regulating IMP formation in fish, the full open reading frame (ORF) of which was 2160bp was obtained for the first time in triploid crucian carp (). It encoded 719 amino acids with a molecular mass of 82.97 kDa, and the theoretical isoelectric point value was 6.31. The homology analysis showed that the homology of triploid crucian carp and diploid as the highest, up to 99%. And the phylogenetic tree showed that triploid crucian carp was grouped with diploid , , and . And real-time fluorescence quantitative results showed that was expressed specifically in muscle of triploid crucian carp ( < 0.05). The results of detection the localization of in cells indicated that the was mainly localized in cytoplasm and cell membrane. Further, we examined the effects of glutamate which was the promotor of IMP formation on the expression of and the formation of IMP and experiments, the results showed that 3% glutamate and 2 mg/ml glutamate could significantly promote expression and IMP formation in triploid crucian carp muscle tissue and muscle cells ( < 0.05). Then we inhibited the expression of and experiments, we found the formation of IMP in muscle tissue and muscle cells of triploid crucian carp all were inhibited and they affected the gene expression of AMPK-mTOR signaling pathway. The all results showed that mediated glutamate through AMPK-mTOR signaling pathway to regulate the formation of fish IMP.