Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China.
Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China.
Int J Biol Macromol. 2022 Dec 1;222(Pt A):325-336. doi: 10.1016/j.ijbiomac.2022.09.108. Epub 2022 Sep 14.
Monochamus saltuarius (Coleoptera: Cerambycidae) was reported as the vector beetle of the pine wood nematode (PWN, Bursaphelenchus xylophilus) in Japan and Europe. It was first reported to transmitted the PWN to native Pinus species in 2018 in Liaoning Province, China. However, the lack of genomic resources has limited the in-depth understanding of its interspecific relationship with PWN. Here, we obtained a chromosome-level reference genome of M. saltuarius combining Illumina, Nanopore and Hi-C sequencing technologies. We assembled the scaffolds into ten chromosomes (including an X chromosome) and obtained a 682.23 Mb chromosome-level genome with a N50 of 73.69 Mb. In total, 427.67 Mb (62.69 %) repeat sequences were identified and 14, 492 protein-coding genes were predicted, of which 93.06 % were annotated. We described the mth/mthl, P450, OBP and OR gene families associated with the vector beetle's development and resistance, as well as the host selection and adaptation, which serve as a valuable resource for understanding the host adaptation in insects during evolution. This high quality reference genome of M. saltuarius also provide new avenues for researching the mechanism of this synergistic damage between vector beetles and PWN.
黄斑幽天牛（鞘翅目：天牛科）在日本和欧洲被报道为松材线虫（松材线虫，Bursaphelenchus xylophilus）的媒介甲虫。它于 2018 年在中国辽宁省首次被报道将松材线虫传播给本地的松树物种。然而，基因组资源的缺乏限制了对其与松材线虫种间关系的深入了解。在这里，我们结合 Illumina、Nanopore 和 Hi-C 测序技术，获得了黄斑幽天牛的染色体水平参考基因组。我们将支架组装成十个染色体（包括一个 X 染色体），并获得了一个 682.23 Mb 的染色体水平基因组，N50 为 73.69 Mb。总共鉴定出 427.67 Mb（62.69%）的重复序列和 14492 个蛋白质编码基因，其中 93.06%被注释。我们描述了与媒介甲虫发育和抗性相关的 mth/mthl、P450、OBP 和 OR 基因家族，以及宿主选择和适应，这为理解昆虫在进化过程中对宿主的适应提供了有价值的资源。黄斑幽天牛的高质量参考基因组也为研究媒介甲虫和松材线虫协同损伤的机制提供了新途径。
