松材线虫携带者单色齿爪鳃金龟的染色体水平基因组组装

Chromosome-level genome assembly of the pine wood nematode carrier Arhopalus unicolor.

作者信息

Zou Xiangying, Zeng Liqiong, You Xingzao, Zhong Jinghui, Tang Chensheng, Zheng Zhaofei, Lin Xiaochun, Zhang Biaoqiang, Zhang Xinliang, Fang Yu, Sun Yanli, Cai Shouping, Su Jun

机构信息

Key Laboratory of State Forestry and Grassland Administration on Timber Forest Breeding and Cultivation for Mountainous Areas in Southern China, Fujian Academy of Forestry, Fuzhou, 350012, China.

Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, College of Life Sciences, Fuzhou, 350002, China.

出版信息

Sci Data. 2025 Jan 20;12(1):111. doi: 10.1038/s41597-025-04460-8.

DOI:10.1038/s41597-025-04460-8

PMID:39833239

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11747178/

Abstract

Arhopalus unicolor is a carrier of the pine wood nematode (PWN), which causes pine wilt disease, killing pine trees and causing considerable economic and environmental losses. While the A. unicolor mitochondrial genome has been published, a high-quality genome assembly and annotation of A. unicolor is not yet available. To address this, we assembled a chromosome-level reference genome assembly of A. unicolor with a combination of Illumina, PacBio, and Hi-C sequencing technologies. The final genome size was determined to be 1268.11 Mb, with a GC% of 32.44%, and the scaffold N50 value was 19.30 Mb. A total of 98.77% of the assembled sequences mapped to 10 pseudochromosomes, and BUSCO analysis revealed high completeness, with 97.15% gene coverage. Furthermore, the genome contains 71.74% repeat elements and encompasses 16,450 predicted protein-coding genes. This genome sequence of A. unicolor will be a valuable resource for understanding the genetics and evolutionary history of this species and for developing effective management strategies for this PWN carrier.

摘要

单色齿小蠹是松材线虫（PWN）的携带者，松材线虫会引发松树枯萎病，导致松树死亡，并造成相当大的经济和环境损失。虽然单色齿小蠹的线粒体基因组已公布，但高质量的单色齿小蠹基因组组装和注释尚未可得。为了解决这一问题，我们结合Illumina、PacBio和Hi-C测序技术，组装了单色齿小蠹的染色体水平参考基因组。最终确定基因组大小为1268.11 Mb，GC含量为32.44%，支架N50值为19.30 Mb。总共98.77%的组装序列映射到10条假染色体上，BUSCO分析显示完整性高，基因覆盖率为97.15%。此外，该基因组包含71.74%的重复元件，涵盖16450个预测的蛋白质编码基因。单色齿小蠹的这一基因组序列将成为了解该物种的遗传学和进化历史以及制定针对这种松材线虫携带者的有效管理策略的宝贵资源。