Wang Ji-Sheng, Dai Heng-Heng, Zhang Kai-Ge, Cao Ke-Gang, Deng Sheng, Bao Bing-Hao, Feng Jun-Long, Meng Fan-Chao, Li Hai-Song, Wang Bin
First Clinical School of Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
Dongzhimen Hospital Affiliated to Beijing University of Traditional Chinese Medicine, Beijing 100700, China.
Chin Herb Med. 2021 Apr 30;13(3):351-358. doi: 10.1016/j.chmed.2021.04.016. eCollection 2021 Jul.
To study the therapeutic effect of Huoxue Tongluo Decoction (HXTLD) on erectile dysfunction caused by ischemic stroke and identify the mechanisms involved.
Network pharmacology was used to predict the key active ingredients and targets of HXTLD. Surgical methods were used to create a rat model of ischemic stroke. The rats were then given a suspension of HXTLD by ig administration. Erectile function was evaluated by Apomorphine (APO) induction. Real-time fluorescence quantitative reverse transcription-polymerase chain reaction (Real-time PCR) and Western blotting were used to detect the expression of related mRNAs and proteins in rat penile corpus cavernous tissue and brain tissue. Hematoxylin & Eosin (HE) staining was used to investigate structural changes in the penile cavernous tissue.
Network pharmacology showed that tumor necrosis factor (TNF), nitric oxide synthase 3 (eNOS), and vascular endothelial growth factor (VEGF) were the key targets of HXTLD in the treatment of erectile dysfunction caused by ischemic stroke. Experimental studies showed that HXTLD improved erectile dysfunction caused by ischemic stroke. HE results showed that HXTLD improved the structure of the corpus cavernosa. HXTLD also inhibited the expression of TNF and VEGF proteins in penile tissue ( < 0.05) and enhanced the expression of eNOS protein in penile tissue ( < 0.05).
HXTLD improved the erectile function of rats with erectile dysfunction caused by ischemic stroke by regulating the mRNA and protein levels of TNF, eNOS and VEGF.
研究活血通络汤（HXTLD）对缺血性脑卒中所致勃起功能障碍的治疗作用，并明确其相关机制。
采用网络药理学预测HXTLD的关键活性成分和靶点。运用手术方法建立大鼠缺血性脑卒中模型。然后通过灌胃给予大鼠HXTLD混悬液。采用阿扑吗啡（APO）诱导法评估勃起功能。运用实时荧光定量逆转录-聚合酶链反应（Real-time PCR）和蛋白质印迹法检测大鼠阴茎海绵体组织和脑组织中相关mRNA和蛋白质的表达。采用苏木精-伊红（HE）染色观察阴茎海绵体组织的结构变化。
网络药理学研究显示，肿瘤坏死因子（TNF）、一氧化氮合酶3（eNOS）和血管内皮生长因子（VEGF）是HXTLD治疗缺血性脑卒中所致勃起功能障碍的关键靶点。实验研究表明，HXTLD改善了缺血性脑卒中所致的勃起功能障碍。HE染色结果显示，HXTLD改善了海绵体结构。HXTLD还抑制了阴茎组织中TNF和VEGF蛋白的表达（<0.05），并增强了阴茎组织中eNOS蛋白的表达（<0.05）。
HXTLD通过调节TNF、eNOS和VEGF的mRNA和蛋白质水平，改善了缺血性脑卒中所致勃起功能障碍大鼠的勃起功能。
