Department of Stomatology, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, China.
State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shaanxi Key Laboratory of Oral Diseases, Department of Operative Dentistry and Endodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, China.
Biomed Mater Eng. 2023;34(1):95-109. doi: 10.3233/BME-221426.
Platelet lysate (PL) is considered as an alternative to fetal bovine serum (FBS) and facilitates the proliferation and differentiation of mesenchymal cells.
The aim of this study is to explore whether super activated platelet lysate (sPL), a novel autologous platelet lysate, has the ability to inhibit inflammation and promote cell proliferation, repair and osteogenesis as a culture medium.
Different concentrations of sPL on human fetal osteoblastic 1.19 cell line (hFOB1.19) proliferation and apoptotic repair were investigated; And detected proliferative capacity, inflammatory factor expressions and osteogenic differentiation of human dental pulp cells (hDPCs) stimulated by LPS under 10% FBS and 5% sPL mediums.
sPL promoted hFOB1.19 proliferation and had repairing effects on apoptotic cells. No significant difference in proliferation and IL-1α, IL-6 and TNF-α expressions of hDPCs in FBS and sPL medium stimulated by LPS. hDPCs in sPL osteogenic medium had higher osteogenic-related factor expressions and ALP activity. LPS promoted osteogenic-related factor expressions and ALP activity of hDPCs in FBS osteogenic medium, but opposite effect showed in sPL medium.
sPL promoted osteoblast proliferation and had restorative effects. Under LPS stimulation, sPL did not promote hDPCs proliferation or inhibit inflammation. sPL promotes osteogenic differentiation of hDPCs.
血小板裂解液（PL）被认为是胎牛血清（FBS）的替代品，能够促进间充质细胞的增殖和分化。
本研究旨在探索新型自体血小板裂解液（sPL）是否具有作为培养基抑制炎症、促进细胞增殖、修复和成骨的能力。
研究不同浓度 sPL 对人胚骨原代细胞（hFOB1.19）增殖和细胞凋亡修复的影响；并检测 LPS 刺激下人牙髓细胞（hDPCs）在 10% FBS 和 5% sPL 培养基中的增殖能力、炎症因子表达和成骨分化。
sPL 促进 hFOB1.19 增殖，对凋亡细胞具有修复作用。LPS 刺激下，FBS 和 sPL 培养基中 hDPCs 的增殖及 IL-1α、IL-6 和 TNF-α 表达无显著差异。sPL 成骨培养基中的 hDPCs 具有更高的成骨相关因子表达和 ALP 活性。LPS 促进 FBS 成骨培养基中 hDPCs 的成骨相关因子表达和 ALP 活性，但在 sPL 培养基中则表现出相反的效果。
sPL 促进成骨细胞增殖，具有修复作用。在 LPS 刺激下，sPL 不会促进 hDPCs 增殖或抑制炎症。sPL 促进 hDPCs 的成骨分化。
