Meng Ling-Qi, Zhang Yi, Liu Chun-Xiang, Ullah Ihsan, Zhang Tian-Qi, Zhang Yi
National and Local Joint Stem Cell Research and Engineering Center for Aging Diseases, Tian Qing Stem Cell Co. Ltd, Harbin, Heilongjiang, 150028, China.
Laboratory of Basic and Application Research of Bei yao, Heilongjiang University of Chinese Medicine, Ministry of Education, 24 Heping Road, Harbin, Heilongjiang, 150040, China.
Sci Rep. 2025 Jul 9;15(1):24698. doi: 10.1038/s41598-025-10587-w.
This study aimed to establish a rat model of thin endometrium and investigate the effects of super-activated platelet lysate (sPL) and umbilical cord mesenchymal stem cells (UCMSCs) on the thin endometrium in rats. Thin endometrium models were induced by infusing absolute ethyl alcohol into the uteri of female Sprague-Dawley (SD) rats. Rats were randomly assigned to several groups (Control, Model, Extracellular matrix (ECM) + sPL, ECM + cell, Gel + sPL, Gel + cell) and treated for 21 or 42 days. Histopathological structures and endometrial thickness were observed using hematoxylin-eosin (HE) staining. ELISA was used to detect PDGF-BB, TGF-β1, E2 and FSH expression levels in serum. Furthermore, Western blot and immunohistochemical staining were used to assess the expression levels of cyclin D1, CD34, pan-keratin, cytokeratin 18, and vimentin in uterine tissue. HE staining revealed improvements in endometrial thickness, gland number, and blood vessels following treatment with sPL and UCMSCs in the thin endometrium rat model. Compared to the model group, ELISA results demonstrated that the PDGF-BB, E2, TGF-β1 and FSH serum in treatment groups returned to control levels. Immunohistochemical staining and Western blot results indicated decreased keratin, cytokeratin, and vimentin expression levels in the model group, which were significantly increased by sPL perfusion or UCMSCs transplantation. Intrauterine perfusion of sPL and UCMSCs improves endometrium thickness, morphology, function, and repair capacity in rats with thin endometrium.
本研究旨在建立薄型子宫内膜大鼠模型，并研究超活化血小板裂解液（sPL）和脐带间充质干细胞（UCMSCs）对大鼠薄型子宫内膜的影响。通过向雌性Sprague-Dawley（SD）大鼠子宫内注入无水乙醇诱导建立薄型子宫内膜模型。将大鼠随机分为几组（对照组、模型组、细胞外基质（ECM）+sPL组、ECM+细胞组、凝胶+sPL组、凝胶+细胞组），并进行21天或42天的治疗。采用苏木精-伊红（HE）染色观察组织病理学结构和子宫内膜厚度。采用酶联免疫吸附测定（ELISA）检测血清中血小板衍生生长因子-BB（PDGF-BB）、转化生长因子-β1（TGF-β1）、雌二醇（E₂）和促卵泡生成素（FSH）的表达水平。此外，采用蛋白质免疫印迹法（Western blot）和免疫组织化学染色评估子宫组织中细胞周期蛋白D1、CD34、泛角蛋白、细胞角蛋白18和波形蛋白的表达水平。HE染色显示，在薄型子宫内膜大鼠模型中，sPL和UCMSCs治疗后子宫内膜厚度、腺体数量和血管有所改善。ELISA结果表明，与模型组相比，治疗组血清中的PDGF-BB、E₂、TGF-β1和FSH恢复到对照水平。免疫组织化学染色和Western blot结果表明，模型组角蛋白、细胞角蛋白和波形蛋白表达水平降低，而sPL灌注或UCMSCs移植可使其显著升高。子宫内灌注sPL和UCMSCs可改善薄型子宫内膜大鼠的子宫内膜厚度、形态、功能和修复能力。
