Jung Soo-Bin, Kang Min-Soo, Jung Joo-Young, Kwon Jong-Hee
Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 52828, Republic of Korea.
Division of Applied Life Sciences (BK21), Gyeongsang National University, Jinju, 52828, Republic of Korea.
Bioprocess Biosyst Eng. 2022 Oct;45(10):1731-1738. doi: 10.1007/s00449-022-02781-1. Epub 2022 Sep 19.
Phycocyanin (PC) is a natural blue pigment that has great commercial value in food and pharmaceutical industry. Arthrospira (Spirulina) platensis is a photosynthetic spiral-shaped cyanobacterium containing a rich PC pigment. Autolysis is the enzymatic digestion of cells by the action of its own enzymes. To develop an effective and economical extraction process, an autolysis process was incorporated into the conventional freezing-thawing method. In the present study, 91% of maximal extraction yield of PC with 1.194 purity (A/A) was obtained via autolysis after 3 h of incubation at 37 °C without using an extraction salt solution or a successive freezing-thawing process. In addition to temperature, the initial concentration of bicarbonate in growth medium and the concentration of wet biomass are important parameters that influence the extraction yield of PC by autolysis.
藻蓝蛋白（PC）是一种天然蓝色色素，在食品和制药行业具有巨大的商业价值。钝顶节旋藻是一种光合螺旋形蓝细菌，含有丰富的藻蓝蛋白色素。自溶是细胞通过自身酶的作用进行的酶促消化。为了开发一种有效且经济的提取工艺，将自溶工艺纳入传统的冻融法中。在本研究中，在37℃孵育3小时后，通过自溶获得了纯度为1.194（A/A）的藻蓝蛋白最大提取产率的91%，且无需使用提取盐溶液或连续冻融工艺。除温度外，生长培养基中碳酸氢盐的初始浓度和湿生物质浓度是影响自溶法提取藻蓝蛋白产率的重要参数。
