利用油废料通过毕赤酵母 GS115 工程菌及发酵罐生产真菌 GLA。

Engineering and fermenter production of fungi GLA in Pichia pastoris GS115 using oil waste.

机构信息

Department of Biology, Yazd University, Yazd, 89195-74, Iran.

Department of Biochemistry, Tarbiat Modares University, Tehran, 14115-175, Iran.

出版信息

Arch Microbiol. 2022 Sep 20;204(10):635. doi: 10.1007/s00203-022-03182-4.

DOI:10.1007/s00203-022-03182-4

PMID:36127512

Abstract

γ-Linolenic acid (GLA) is an essential n-6 polyunsaturated fatty acid (PUFA) that has received considerable attention in human and animal feed. GLA is used in many nutritional and medicinal applications, such as the treatment of cancer, inflammatory disorders, and diabetes. Currently, plant seed is the primary dietary source of GLA that is not enough to utilize on an industrial scale. To generate a sustainable novel source of GLA, the gene of delta-6 desaturase, one of the essential enzymes in the GLA production pathway, was isolated from Mucor rouxii DSM1194 and expressed in P. pastoris GS115 by pPICZC vector. The recombinant yeast expressed the GLA up to 19.2% (72 mg/g) of total fatty acids. GLA production of recombinant yeast was studied in a fermenter by oil waste for 5 days, and results detected 6.3 g/l lipid, and 103 mg/g GLA was produced in 72 h. The present study may provide an opportunity to develop an alternative host for manufacturing GLA on an industrial scale.

摘要

γ-亚麻酸（GLA）是一种必需的 n-6 多不饱和脂肪酸（PUFA），在人类和动物饲料中受到广泛关注。GLA 可用于许多营养和药用应用，如治疗癌症、炎症性疾病和糖尿病。目前，植物种子是 GLA 的主要膳食来源，但不足以在工业规模上利用。为了生成一种可持续的新型 GLA 来源，我们从 Mucor rouxii DSM1194 中分离出 GLA 生产途径中的关键酶 delta-6 去饱和酶的基因，并通过 pPICZC 载体在 P. pastoris GS115 中表达。重组酵母将 GLA 表达到总脂肪酸的 19.2%（72mg/g）。我们在发酵罐中用废油对重组酵母进行了 5 天的 GLA 生产研究，结果在 72 小时内检测到 6.3g/l 脂质和 103mg/g 的 GLA。本研究可能为在工业规模上制造 GLA 提供了一种替代宿主的机会。