Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, 430062, PR China.
J Microbiol. 2011 Feb;49(1):151-4. doi: 10.1007/s12275-011-0254-7. Epub 2011 Mar 3.
The delta 6-desaturase gene was cloned from Rhizopus stolonifer, which could accumulate up to 49% of gamma-linolenic acid (GLA, C18:3 Δ(6,9,12)) to the total fatty acids. The cloned DNA contains a 1,380 bp open reading frame encoding a protein of 460 amino acids, which showed high similarity to those of fungal delta 6-desaturases with three conserved histidine-rich motifs and HPGG motif. Notably, this deduced sequence had a shorter C-terminus. Results demonstrated that the cDNA sequence exhibited delta 6-desaturase activity by accumulation of about 22.4 % of GLA to the total fatty acids in the recombinant Pichia pastoris strain GS115.
从根霉（Rhizopus stolonifer）中克隆了 delta 6-去饱和酶基因，该基因可将高达 49%的 γ-亚麻酸（GLA，C18:3 Δ(6,9,12))积累到总脂肪酸中。克隆的 DNA 包含一个 1380bp 的开放阅读框，编码一个 460 个氨基酸的蛋白质，该蛋白质与真菌 delta 6-去饱和酶具有高度相似性，具有三个保守的富含组氨酸的基序和 HPGG 基序。值得注意的是，这个推导的序列具有较短的 C 末端。结果表明，该 cDNA 序列通过在重组毕赤酵母（Pichia pastoris）菌株 GS115 中积累约 22.4%的 GLA 到总脂肪酸中表现出 delta 6-去饱和酶活性。
