Watanabe S, Shirakami A, Takeichi T, Ohara T, Saito S
J Clin Lab Immunol. 1987 May;23(1):45-51.
The numbers of total lymphocyte and lymphocyte subsets in peripheral blood of patients with silicosis and their serum immunoglobulin (Ig) levels were studied to evaluate their immune status. The numbers of total lymphocytes and OKT4+ and OKT8+ cells tended to be decreased and the numbers of OKT3+ cells was significantly decreased (p less than 0.001), but that of OKIa-1+ cells was increased (p less than 0.001) in the patients. In the lymph nodes of the lung hilus, the ratio of OKT4+ cells to total lymphocytes was increased but that of OKT8+ cells to total lymphocytes was decreased. The serum levels of IgG and IgA were elevated in the patients with reduction in the numbers of total lymphocytes and OKT4+ cells in peripheral blood. The serum IgE levels in 17 of 82 patients were above 400 IU/ml. In 8 patients with serum IgE 82 patients were above 400 IU/ml. In 8 patients with serum IgE levels of more than 1,000 IU/ml, the numbers of total lymphocytes and OKT3+ and OKT4+ cells were decreased compared with those in controls. The following suggestions are proposed from the results: Increase and activation of helper T cells may be caused by the presentation of silica dust to macrophages in the lymph nodes, and these cells may then stimulate B cell proliferation and Ig production by B cells, resulting in increase in the serum Ig (IgG, IgA and IgE) level. Reduction in the number of OKT4+ cells helper/inducer T cells) in peripheral blood may be due to migration of helper T cells into the lymph nodes. These immunological events in patients with silicosis are probably due to the adjuvant effect of silica dust.
为评估矽肺患者的免疫状态，研究了其外周血中总淋巴细胞及淋巴细胞亚群数量以及血清免疫球蛋白（Ig）水平。患者外周血中总淋巴细胞、OKT4⁺和OKT8⁺细胞数量呈减少趋势，OKT3⁺细胞数量显著减少（p＜0.001），但OKIa - 1⁺细胞数量增加（p＜0.001）。在肺门淋巴结中，OKT4⁺细胞与总淋巴细胞的比例增加，而OKT8⁺细胞与总淋巴细胞的比例降低。外周血中总淋巴细胞和OKT4⁺细胞数量减少的患者血清IgG和IgA水平升高。82例患者中有17例血清IgE水平高于400 IU/ml。8例血清IgE水平超过1000 IU/ml的患者，其总淋巴细胞、OKT3⁺和OKT4⁺细胞数量与对照组相比减少。根据结果提出以下建议：二氧化硅粉尘在淋巴结中呈递给巨噬细胞可能导致辅助性T细胞增加和激活，这些细胞随后可能刺激B细胞增殖及B细胞产生Ig，导致血清Ig（IgG、IgA和IgE）水平升高。外周血中OKT4⁺细胞（辅助/诱导性T细胞）数量减少可能是由于辅助性T细胞迁移至淋巴结。矽肺患者的这些免疫事件可能归因于二氧化硅粉尘的佐剂作用。
