Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA.
Mayo Clinic, Rochester, MN, USA.
Methods Mol Biol. 2022;2546:365-373. doi: 10.1007/978-1-0716-2565-1_32.
Parathyroid hormone (PTH), an 84-amino acid peptide hormone, is a major regulator of calcium homeostasis. Quantification of PTH in serum is used clinically to investigate calcium imbalances and for monitoring osteodystrophy in patients with renal failure. In addition to intact PTH, several PTH fragments are found in circulation. Recent studies have shown that accurate quantification of PTH fragments may provide valuable clinical information in certain scenarios. In this chapter, a high-resolution mass spectrometry-based method for quantification of PTH (1-84) and its fragments is described. This method involves immunoaffinity capture of intact PTH and PTH-fragments followed by liquid chromatography-high-resolution mass spectrometry (LC-HRMS).
甲状旁腺激素（PTH）是一种由 84 个氨基酸组成的肽类激素，是钙稳态的主要调节剂。血清中 PTH 的定量检测临床上用于研究钙失衡和监测肾衰竭患者的骨营养不良。除了完整的 PTH 外，循环中还存在几种 PTH 片段。最近的研究表明，准确测定 PTH 片段可能在某些情况下提供有价值的临床信息。本章介绍了一种基于高分辨率质谱的 PTH（1-84）及其片段定量方法。该方法包括完整 PTH 和 PTH 片段的免疫亲和捕获，然后进行液相色谱-高分辨率质谱（LC-HRMS）分析。
