MacLeod A R, Talbot K, Smillie L B, Houlker C
J Mol Biol. 1987 Mar 5;194(1):1-10. doi: 10.1016/0022-2836(87)90710-8.
We have isolated a cDNA that contains the complete coding sequence of a 3.0 X 10(3) base human fibroblast mRNA together with a large part of its 3' untranslated sequence. The deduced protein sequence is very similar if not identical to the sequence of horse platelet tropomyosin, a 247 amino acid protein. In vitro translation of an SP6 transcript of this cDNA reveals that the protein product of the 3.0 X 10(3) base mRNA is TM30p1, one of the five proteins in human fibroblasts that have been shown to possess the physical and chemical characteristics of tropomyosin. This mRNA is encoded by a gene family that consists of a functional gene and multiple RNA-copy pseudogenes. This family of sequences is distinct from the gene family encoding TM30nm, a cytoskeletal tropomyosin very similar in electrophoretic mobility to TM30p1, but which shows significant differences in primary structure.
我们分离出了一个cDNA，它包含一个3.0×10³碱基的人成纤维细胞mRNA的完整编码序列及其大部分3'非翻译序列。推导的蛋白质序列即便与马血小板原肌球蛋白（一种247个氨基酸的蛋白质）的序列不完全相同，也非常相似。对该cDNA的SP6转录本进行体外翻译表明，3.0×10³碱基mRNA的蛋白质产物是TM30p1，它是已被证明具有原肌球蛋白物理和化学特性的人成纤维细胞中的五种蛋白质之一。该mRNA由一个基因家族编码，该基因家族由一个功能基因和多个RNA拷贝假基因组成。这个序列家族与编码TM30nm的基因家族不同，TM30nm是一种细胞骨架原肌球蛋白其电泳迁移率与TM30p1非常相似，但在一级结构上存在显著差异。
