Anticancer and antibacterial flavonoids from the callus of ; a new weapon to mitigate the proliferation of cancer cells and bacteria.

A new flavonoid angelioue (1) together with five known compounds cuminatanol (2), myricetin (3), epigallocatechin (4), taxifolin (5) and dihydromyricetin (6) was isolated from the callus extract of (Hand.-Mazz.) W. T. Wang and the structures were elucidated based on their detailed spectroscopic data. Among the compounds, the new compound angelioue (1) displayed significant antibacterial activity against methicillin-resistant (MRSA) with the MIC value of 6.68 μg mL and MBC value of 53.42 μg mL; in contrast the other compounds showed moderate to no antibacterial activity. In addition, known dihydromyricetin (6) exhibited potent cytotoxic activities against mouse breast cancer cells (4T1), human lung adenocarcinoma (A549) and human non-small cell lung cancer (NCI-H1975) tumor cell lines with GI values of 17.47, 18.91 and 20.50 μM mL, respectively. The compounds 1-5 exhibited low micro-molar inhibitory activities. Moreover, the structure-activity relationships of the most active compounds for antibacterial and cytotoxic activities are discussed. The present findings clearly suggest that the callus is a good source of bioactive compounds.