Lai Wenqing, Qiu Yuqin, Pan Shengnan, Wan Aijun, Wang Xueyang, Lv Dingding, Dai Lishang
Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China.
The Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of Agricultural Science, Zhenjiang, China.
Front Immunol. 2025 May 21;16:1584985. doi: 10.3389/fimmu.2025.1584985. eCollection 2025.
Vine tea () is a widely used Chinese herb with a long history of application in southern China, known for its notable anti-infective, antibacterial and immunomodulatory pharmacological properties. It has potential for application in aquaculture as an inexpensive and readily available dietary supplement, promoting growth, antioxidant activity, and immune regulation in fish. However, there have been very few studies investigating the effects of vine tea on fish miRNAs. Loach is an economically important freshwater fish species, highly valued for its delicious flavor, but research on its miRNA relatively limited. To examine immune-related miRNAs in loach and to further determine the antibacterial immune mechanisms of vine tea, we performed small RNA sequencing analysis of loach liver tissue before and after treatment with vine tea extract. The results showed that vine tea could affect the antibacterial immune activity of loach through the miRNA regulation. A total of 25 differentially expressed miRNAs were identified in liver tissues, and KEGG pathway analysis revealed that most of these miRNAs were involved in innate immune responses such as autophagy, lysosomes, endocytosis, and pattern receptor signaling pathways. To the best of our knowledge, this is the first study to profile miRNA expression in loach after treatment with vine tea extract. This work deepens the understanding of the role of loach miRNAs in the immune system and opens new prospects for application of vine tea in aquaculture.
藤茶是一种在中国南方有着悠久应用历史的常用中草药，以其显著的抗感染、抗菌和免疫调节药理特性而闻名。它作为一种廉价且易于获得的膳食补充剂在水产养殖中具有应用潜力，可促进鱼类生长、抗氧化活性和免疫调节。然而，很少有研究调查藤茶对鱼类微小RNA（miRNA）的影响。泥鳅是一种具有重要经济价值的淡水鱼类，因其美味的口感而备受重视，但其miRNA的研究相对有限。为了检测泥鳅中与免疫相关的miRNA，并进一步确定藤茶的抗菌免疫机制，我们对用藤茶提取物处理前后的泥鳅肝脏组织进行了小RNA测序分析。结果表明，藤茶可通过miRNA调控影响泥鳅的抗菌免疫活性。在肝脏组织中总共鉴定出25个差异表达的miRNA，KEGG通路分析显示这些miRNA大多参与自噬、溶酶体、内吞作用和模式识别受体信号通路等固有免疫反应。据我们所知，这是第一项研究藤茶提取物处理后泥鳅中miRNA表达谱的研究。这项工作加深了对泥鳅miRNA在免疫系统中作用的理解，并为藤茶在水产养殖中的应用开辟了新前景。
