Esmeryan Karekin D, Rangelov Ivaylo, Chaushev Todor A
Acoustoelectronics Laboratory, Georgi Nadjakov Institute of Solid State Physics, Bulgarian Academy of Sciences 72, Tzarigradsko Chaussee Blvd. 1784 Sofia Bulgaria
Research Department, Medical Center Neovitro OOD 20, Petko Y. Todorov Blvd. 1408 Sofia Bulgaria.
Nanoscale Adv. 2022 Apr 23;4(13):2806-2815. doi: 10.1039/d2na00192f. eCollection 2022 Jun 28.
Sperm cryopreservation is vital in combating the human infertility, but regrettably, the toxicity of cryoprotectants and the occurrence of intracellular icing, osmotic shocks or shrinkage of the cells below a given threshold volume greatly affects the success rate of this technique. Using the virtue of nanotechnologies and depositing water-repellent soot nanoparticles on the inner walls of cryovials may outline new directions in the development of cryobiology, but doubts related to the soot's venomosity question its practical implementability. The scientific content of this article eliminates the existing apprehensions by analyzing the cytotoxicity of three types of rapeseed oil soot, differing in morphology, surface chemistry and zeta potential, towards human spermatozoa. Upon intermittent evaluations of the sperm motility within 270 min of incubation in vials comprising carbon nanoparticles, we reveal that this soot category is non-cytotoxic or at worst, faintly toxic to the gametes provided by twenty individuals. Enhanced progressive sperm motility is observed at ∼50-60% of patients following the soot treatments, which is attributed to electrostatic repulsions and biochemical alterations in the seminal plasma. These fascinating results open new horizons for incorporation of the rapeseed oil soot as a tool for functional preparation and activation of human spermatozoa preceding fertilization.
精子冷冻保存对于对抗人类不育至关重要，但遗憾的是，冷冻保护剂的毒性以及细胞内结冰、渗透冲击或细胞体积缩小至给定阈值以下的情况，极大地影响了该技术的成功率。利用纳米技术的优势，在冻存管内壁沉积疏水碳烟纳米颗粒，可能为低温生物学的发展勾勒出新方向，但与碳烟毒性相关的疑虑质疑了其实际可实施性。本文的科学内容通过分析三种形态、表面化学和zeta电位不同的菜籽油碳烟对人类精子的细胞毒性，消除了现有的担忧。在含有碳纳米颗粒的小瓶中孵育270分钟期间对精子活力进行间歇性评估后，我们发现这种碳烟类别对20名个体提供的配子无细胞毒性，或者最坏的情况下仅有轻微毒性。在接受碳烟处理的患者中，约50 - 60%观察到精子进行性活力增强，这归因于精浆中的静电排斥和生化改变。这些引人入胜的结果为将菜籽油碳烟用作受精前人类精子功能制备和激活的工具开辟了新视野。
