微量精子 Stripper 管冷冻保存：全球首例活产报告。
Cryopreservation of small numbers of human spermatozoa in a Stripper tip: Report of the first live-birth worldwide.
机构信息
Department of Reproductive Biology, Hôpitaux Universitaires Paris Seine Saint-Denis, AP-HP, 93140, Bondy, France; University Paris XIII, Sorbonne Paris Cité, UFR SMBH, 93000, Bobigny, France.
Department of Reproductive Biology, Hôpitaux Universitaires Paris Seine Saint-Denis, AP-HP, 93140, Bondy, France.
出版信息
Cryobiology. 2021 Apr;99:103-105. doi: 10.1016/j.cryobiol.2021.01.005. Epub 2021 Jan 2.
PURPOSE
The aim is to report the first live-birth following ICSI using spermatozoa previously vitrified in a Stripper Tip.
PRINCIPAL RESULTS
A 34-year-old cryptozoospermic man was enrolled in a sperm vitrification program. After failure of conventional freezing technique, spermatozoa were vitrified using two carriers: a commercially-available, Cell Sleeper, and a "home-made" one, Stripper Tip. This man and his 30-year-old wife underwent an ICSI attempt using vitrified-warmed spermatozoa from these devices. All frozen-warmed spermatozoa were quickly recovered. Among the oocytes retrieved, six were injected with sperm from the Cell Sleeper, and seven with sperm from the Stripper tip, leading to 4 embryos in each case. Two embryos, arising from sperm frozen in the Stripper tip, were transferred, resulting in a healthy live-birth.
CONCLUSIONS
This is the first successful delivery following the use of spermatozoa frozen in an original device, the Stripper Tip, giving a promising prospect for managing severe male infertilities.
目的
报告首例使用 Stripper Tip 冷冻保存的精子进行卵胞浆内单精子注射（ICSI）后的活产婴儿。
主要结果
一名 34 岁的隐匿型精子症患者参加了精子冷冻保存计划。在常规冷冻技术失败后，使用两种载体（一种是市售的 Cell Sleeper，另一种是自制的 Stripper Tip）对精子进行了冷冻。这名男子和他 30 岁的妻子使用这些设备中解冻的冷冻精子进行了 ICSI 尝试。所有冷冻-解冻的精子都很快被回收。在取回的卵母细胞中，有 6 个用 Cell Sleeper 中的精子进行了注射，7 个用 Stripper tip 中的精子进行了注射，每个情况都产生了 4 个胚胎。两个胚胎是用 Stripper tip 中冷冻的精子移植的，最终成功分娩了一个健康的活产婴儿。
结论
这是首例使用原始设备 Stripper Tip 冷冻保存的精子成功分娩的案例，为治疗严重男性不育症提供了有前景的方法。
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