Wang Ju, Wang Lei, Shi Shengnan, Cao Yaqi, Feng Junmei, Liu Changhong, Zheng Lei
Engineering Research Center of Bio-Process, Ministry of Education, School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, China.
Intelligent Interconnected Systems Laboratory of Anhui Province, Hefei University of Technology, Hefei, 230009, China.
Food Funct. 2022 Oct 17;13(20):10476-10490. doi: 10.1039/d2fo01511k.
Probiotic-based therapy is a promising approach, which can positively modulate bacterial composition and maintain homeostasis. However, exogenous probiotics are easily destroyed by harsh conditions ; thus, their application prospects have been severely limited. Specifically, oxytetracycline (OTC), a broad-spectrum antibiotic widely used in aquaculture, results in adverse intestinal environments, such as dysbacteriosis, oxidative damage, and gut dysmotility. Here, we describe a facile method to apply glycol chitosan/alginate as armor on the surface of probiotics to effectively protect them from the changed enteric environments induced by OTC. The results demonstrated that the coated GG (LGG) for only 2 h administration could significantly improve the colonization rate of LGG, and the relative abundance of can reach 80% in OTC-treated larvae intestines. We also explored the specific mechanisms of the coated LGG to diminish reactive oxygen species (ROS) generation and rescue gut dysmotility for OTC treatment, including enhancing the activity of antioxidative enzymes (CAT, SOD and GPx) and increasing 5-HT synthesis. The mitigation effect of the coated LGG for 2 h administration was comparable to that of uncoated LGG for 24 h administration. Encapsulation of LGG with polysaccharides provides a unique application example for generating useful bacterial therapeutics in harsh intestinal environments.
基于益生菌的疗法是一种很有前景的方法，它可以积极调节细菌组成并维持体内平衡。然而，外源性益生菌很容易被恶劣条件破坏；因此，它们的应用前景受到了严重限制。具体而言，土霉素（OTC）是一种广泛用于水产养殖的广谱抗生素，会导致不良的肠道环境，如菌群失调、氧化损伤和肠道运动障碍。在此，我们描述了一种简便的方法，即将壳聚糖二醇/海藻酸盐作为益生菌表面的保护层，以有效保护它们免受OTC诱导的肠道环境变化的影响。结果表明，仅给药2小时的包衣鼠李糖乳杆菌（LGG）就能显著提高LGG的定植率，在经OTC处理的幼虫肠道中其相对丰度可达80%。我们还探究了包衣LGG减少活性氧（ROS）生成并挽救OTC治疗导致的肠道运动障碍的具体机制，包括增强抗氧化酶（CAT、SOD和GPx）的活性以及增加5-羟色胺的合成。给药2小时的包衣LGG的缓解效果与未包衣LGG给药24小时的效果相当。用多糖包裹LGG为在恶劣肠道环境中产生有效的细菌疗法提供了一个独特的应用实例。
