Engineering Research Center of Bio-Process, Ministry of Education, School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, China.
Intelligent Interconnected Systems Laboratory of Anhui Province, Hefei University of Technology, Hefei, 230009, China.
World J Microbiol Biotechnol. 2022 Sep 14;38(12):222. doi: 10.1007/s11274-022-03409-y.
Intestinal peristalsis is essential for gastrointestinal function, which could maintain the appropriate progression and digestion of food and reduce bacterial aggregation through mixing function. Even though certain ingredients of foodstuff are known to increase or decrease intestinal peristalsis, the role of environmental pollutants on intestinal peristalsis is relatively unknown. Therefore, the effects of four typical environmental pollutants (oxytetracycline, arsenic, polychlorinated biphenyls and chlorpyrifos) on intestinal peristalsis in the zebrafish model and then tested the recovery effect of the constipation-resistant probiotic. The results showed that 4-day environmental pollutants exposures on the zebrafish embryos at 1 day post fertilization clearly decreased the intestinal peristalsis through decreasing the serotonin (5-HT) production and down-regulating the expression of key genes involved in 5-HT synthesis. Pollutants-evoked change of gut motility could be normalized in the presence of Lactobacillus rhamnosus GG (LGG) via increasing 5-HT secretion. Exogenous 5-hydroxytryptophan (100 µg/L) could also rescue the dysfunction of gut motility in pollutants-treated zebrfish. The data identified that LGG normalized disorder of intestinal peristalsis induced by environmental pollutants through increasing 5-HT level. The stimulant effect of LGG on peristalsis may be associated with 5-HT system, which could provide references for the application of probiotics in regulation of gut dysmotility.
肠道蠕动对于胃肠道功能至关重要，它可以通过混合功能维持食物的适当推进和消化，减少细菌聚集。尽管某些食物成分已知会增加或减少肠道蠕动，但环境污染物对肠道蠕动的作用相对未知。因此，本研究采用斑马鱼模型研究了四种典型环境污染物（土霉素、砷、多氯联苯和毒死蜱）对肠道蠕动的影响，然后测试了抗便秘益生菌的恢复效果。结果表明，在受精后 1 天的斑马鱼胚胎中，4 天的环境污染物暴露会通过减少 5-羟色胺（5-HT）的产生和下调参与 5-HT 合成的关键基因的表达，明显降低肠道蠕动。通过增加 5-HT 分泌，鼠李糖乳杆菌 GG（LGG）可以使污染物引起的肠道运动变化正常化。外源性 5-羟色氨酸（100μg/L）也可以挽救污染物处理的斑马鱼中肠道运动功能障碍。数据表明，LGG 通过增加 5-HT 水平使环境污染物引起的肠道蠕动紊乱正常化。LGG 对蠕动的刺激作用可能与 5-HT 系统有关，这可为益生菌在调节肠道动力障碍中的应用提供参考。
