In Silico Characterization and Gene Expression Analysis of Toll Signaling Pathway-Related Genes in .

The Asian citrus psyllid, is the main vector of citrus greening disease, also known as Huanglongbing (HLB). Currently, mitigating HLB depends on the control of using insecticides. To design innovative control strategies, we should investigate various biological aspects of at the molecular level. Herein we explored the Toll signaling system-related proteins in using in silico analyzes. Additionally, the transcripts of the identified genes were determined in all life stages from eggs to adults. Our findings reveal that genome possesses Toll signaling pathway-related genes similar to the insect model, , with slight differences. These genes include , , , , , , and encoding Cactus, Toll, Myeloid differentiation factor 88, Dorsal related immunity factor/Dorsal, Pelle, Tube, and Spaetzle, respectively. Unlike , in Dorsal, immunity factor and Dorsal are the same protein. In addition, in , Pelle protein possesses a kinase domain, which is absent in Pelle of Gene expression analysis showed the transcript for , , , , , and are maximum in adults, suggesting the immunity increases with maturity. Instead, transcripts were maximal in eggs and adults and minimal in nymphal stages, indicating its role in embryonic development. The overall findings will help in designing pioneering control strategies of based on repressing its immunity by RNAi or CRISPR and combining that with biological control.