Papadopoulou Evgenia-Anna, Giaki Katerina, Angelis Apostolis, Skaltsounis Alexios-Leandros, Aliferis Konstantinos A
Laboratory of Pesticide Science, Department of Crop Science, Agricultural University of Athens, 11855 Athens, Greece.
Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, University of Athens, 15771 Athens, Greece.
Toxics. 2022 Aug 25;10(9):494. doi: 10.3390/toxics10090494.
Pesticides represent a major human input into the ecosystem, posing a serious risk to non-target organisms. Therefore, there is pressure toward the reduction in their use and the discovery of alternative sources of bioactivity. Endophytic microorganisms represent a source of bioactivity, whose potential for plant protection has been recently established. In this context, an olive tree endophytic sp. was isolated, exhibiting superior antifungal activity, mainly attributed to its major surfactin, iturin, and fengycin and the minor gageotetrin and bacilotetrin groups of lipopeptides (LP). Based on the potential of LP and the lack of information on their toxicity to aquatic organisms, we have investigated the toxicity of an LP extract to the model macrophyte L. The extract exhibited low phytotoxicity (EC = 419 μg·mL), and for the investigation of its effect on the plant, GC/EI/MS metabolomics was applied following exposure to sub-lethal doses (EC and EC). Results revealed a general disturbance of plants' biosynthetic capacity in response to LP treatments, with substantial effect on the amino acid pool and the defense mechanism regulated by jasmonate. There are no previous reports on the phytotoxicity of LP to , with evidence supporting their improved toxicological profile and potential in plant protection.
农药是人类向生态系统的主要输入物，对非目标生物构成严重风险。因此，存在减少其使用以及发现生物活性替代来源的压力。内生微生物是生物活性的一个来源，其在植物保护方面的潜力最近已得到证实。在此背景下，分离出一种橄榄树内生菌，它表现出优异的抗真菌活性，这主要归因于其主要的表面活性素、伊枯草菌素和丰原素以及次要的盖奥四肽和芽孢杆菌四肽类脂肽（LP）。基于脂肽的潜力以及缺乏关于其对水生生物毒性的信息，我们研究了一种脂肽提取物对模式大型植物的毒性。该提取物表现出低植物毒性（EC = 419 μg·mL），为了研究其对植物的影响，在暴露于亚致死剂量（EC和EC）后应用了气相色谱/电子轰击/质谱代谢组学方法。结果显示，植物的生物合成能力因脂肽处理而普遍受到干扰，对氨基酸库和由茉莉酸调节的防御机制有重大影响。以前没有关于脂肽对该植物毒性的报道，有证据支持其改善的毒理学特征及其在植物保护中的潜力。
