Papadopoulou Evgenia-Anna, Angelis Apostolis, Antoniadi Lemonia, Aliferis Konstantinos A, Skaltsounis Alexios-Leandros
Laboratory of Pesticide Science, Department of Crop Science, Agricultural University of Athens, 11855 Athens, Greece.
Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, University of Athens, 15771 Athens, Greece.
Metabolites. 2021 Dec 2;11(12):833. doi: 10.3390/metabo11120833.
Endophytic microorganisms (EMs) have recently attracted interest for applications in plant protection, mainly due to their bioactive compound-producing capacity. Therefore, we underwent the task of isolating olive tree EMs and investigating their bioactivity against the devastating pathogen . Several EMs were isolated; however, the sp. PTA13 isolate exhibited the highest toxicity to the phytopathogen. Bacteria of the genus exhibit superior bioactive metabolite-producing capacity, with the lipopeptides (LPs) of surfactin, iturin, and fengycin groups being the most studied. A total LP extract and several fractions were obtained, and their bioactivity was assessed against strains. LPs of the major surfactin, iturin, and fengycin groups and the minor gageotetrin and bacilotetrin groups were annotated. The results confirmed the bioactivity of the major LPs, with fengycins being the most fungitoxic. Interestingly, the minor LP fraction exhibited selective toxicity to the fungicide-resistant isolate, an observation that highlights the significance of our approach to comprehensively mine the total LP extract. This work represents a proof of concept of the exploitation of EMs in customized olive tree plant protection and aligns well with strategies that focus on the sustainability and safety of food production via the development of next-generation plant protection products.
内生微生物（EMs）最近因其在植物保护中的应用而受到关注，主要是由于它们具有产生生物活性化合物的能力。因此，我们承担了分离橄榄树内生微生物并研究其对毁灭性病原菌的生物活性的任务。分离出了几种内生微生物；然而，sp. PTA13分离株对植物病原菌表现出最高的毒性。属的细菌具有卓越的产生生物活性代谢物的能力，其中表面活性素、伊枯草菌素和丰原素组的脂肽（LPs）是研究最多的。获得了总脂肽提取物和几个馏分，并评估了它们对菌株的生物活性。对主要的表面活性素、伊枯草菌素和丰原素组以及次要的加吉菌素和杆菌菌素组的脂肽进行了注释。结果证实了主要脂肽的生物活性，其中丰原素的杀真菌毒性最强。有趣的是，次要脂肽馏分对耐杀菌剂的分离株表现出选择性毒性，这一观察结果突出了我们全面挖掘总脂肽提取物方法的重要性。这项工作代表了在定制橄榄树植物保护中利用内生微生物的概念验证，并且与通过开发下一代植物保护产品来关注粮食生产的可持续性和安全性的策略高度一致。
