Bai Yutao, Henry Jason, Karpiński Tomasz M, Wlodkowic Donald
The Neurotox Lab, School of Science, RMIT University, Plenty Road, P.O. Box 71, Bundoora, VIC 3083, Australia.
Chair and Department of Medical Microbiology, Poznań University of Medical Sciences, Wieniawskiego 3, 61-712 Poznań, Poland.
Toxics. 2022 Aug 29;10(9):508. doi: 10.3390/toxics10090508.
Analysis of sensorimotor behavioral responses to stimuli such as light can provide an enhanced relevance during rapid prioritisation of chemical risk. Due to technical limitations, there have been, however, only minimal studies on using invertebrate phototactic behaviors in aquatic ecotoxicity testing. In this work, we demonstrate an innovative, purpose-built analytical system for a high-throughput phototactic biotest with nauplii of euryhaline brine shrimp . We also, for the first time, present a novel and dedicated bioinformatic approach that facilitates high-throughput analysis of phototactic behaviors at scale with great fidelity. The nauplii exhibited consistent light-seeking behaviors upon extinguishing a brief programmable light stimulus (5500K, 400 lux) without habituation. A proof-of-concept validation involving the short-term exposure of eggs (24 h) and instar I larval stages (6 h) to sub-lethal concentrations of insecticides organophosphate chlorpyrifos (10 µg/L) and neonicotinoid imidacloprid (50 µg/L) showed perturbation in light seeking behaviors in the absence of or minimal alteration in general mobility. Our preliminary data further support the notion that phototactic bioassays can represent an attractive new avenue in behavioral ecotoxicology because of their potential sensitivity, responsiveness, and low cost.
分析对光等刺激的感觉运动行为反应，在快速确定化学风险优先级时可提供更高的相关性。然而，由于技术限制，在水生生态毒性测试中使用无脊椎动物趋光行为的研究极少。在这项工作中，我们展示了一种创新的、专门构建的分析系统，用于对广盐性卤虫无节幼体进行高通量趋光生物测试。我们还首次提出了一种新颖且专用的生物信息学方法，该方法有助于大规模、高保真地对趋光行为进行高通量分析。在熄灭短暂的可编程光刺激（5500K，400勒克斯）后，无节幼体表现出一致的趋光行为，且无习惯化现象。一项概念验证性验证实验，将卵（24小时）和Ⅰ龄幼虫阶段（6小时）短期暴露于亚致死浓度的有机磷杀虫剂毒死蜱（10微克/升）和新烟碱类杀虫剂吡虫啉（50微克/升）中，结果表明，在总体活动能力无变化或变化极小的情况下，趋光行为受到了干扰。我们的初步数据进一步支持了这样一种观点，即趋光生物测定法因其潜在的敏感性、响应性和低成本，可成为行为生态毒理学中一条有吸引力的新途径。
