大鼠脑透析液中儿茶酚和吲哚的高效液相色谱-电化学分析
HPLC-EC analysis of catechols and indoles in rat brain dialysates.
作者信息
Sharp T, Zetterström T, Series H G, Carlsson A, Grahame-Smith D G, Ungerstedt U
出版信息
Life Sci. 1987 Aug 17;41(7):869-72. doi: 10.1016/0024-3205(87)90183-4.
This short article reviews HPLC-EC methodology that we are currently applying to measure DA, 5-HT and their acid metabolites in rat brain dialysates collected in vivo. HPLC-EC systems based on standard-bore HPLC columns are described which are sufficiently sensitive to allow detection of the monoamine transmitters and their metabolites in regional brain dialysates collected at 10-20 min intervals. A large reduction in sample requirement was achieved by "down-scaling" the conventional HPLC-EC assay to incorporate small-bore HPLC columns. The small-bore systems allowed monoamines to be detected in samples collected over 1 to a few minutes.
这篇短文回顾了我们目前用于测量体内采集的大鼠脑透析液中多巴胺（DA）、5-羟色胺（5-HT）及其酸性代谢物的高效液相色谱-电化学检测（HPLC-EC）方法。文中描述了基于标准内径HPLC柱的HPLC-EC系统，该系统灵敏度足够高，能够检测以10 - 20分钟间隔采集的局部脑透析液中的单胺类递质及其代谢物。通过将传统的HPLC-EC分析方法“缩小规模”以纳入小内径HPLC柱，实现了样品需求量的大幅减少。小内径系统能够在1至几分钟内采集的样品中检测到单胺类物质。
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