海蛇尾腕再生过程中的神经发生以一组保守的关键发育基因为特征。

Neurogenesis during Brittle Star Arm Regeneration Is Characterised by a Conserved Set of Key Developmental Genes.

作者信息

Czarkwiani Anna, Taylor Jack, Oliveri Paola

机构信息

Department of Genetics, Evolution and Environment, University College London, Darwin Building, Gower Street, London WC1E 6BT, UK.

Center for Regenerative Therapies Dresden (CRTD), Technische Universität (TU) Dresden, 01307 Dresden, Germany.

出版信息

Biology (Basel). 2022 Sep 16;11(9):1360. doi: 10.3390/biology11091360.

DOI:10.3390/biology11091360

PMID:36138839

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9495562/

Abstract

Neural regeneration is very limited in humans but extremely efficient in echinoderms. The brittle star can regenerate both components of its central nervous system as well as the peripheral system, and understanding the molecular mechanisms underlying this ability is key for evolutionary comparisons not only within the echinoderm group, but also wider within deuterostomes. Here we characterise the neural regeneration of this brittle star using a combination of immunohistochemistry, in situ hybridization and Nanostring nCounter to determine the spatial and temporal expression of evolutionary conserved neural genes. We find that key genes crucial for the embryonic development of the nervous system in sea urchins and other animals are also expressed in the regenerating nervous system of the adult brittle star in a hierarchic and spatio-temporally restricted manner.

摘要

神经再生在人类中非常有限，但在棘皮动物中极其高效。脆星能够再生其中枢神经系统的两个组成部分以及外周系统，了解这种能力背后的分子机制不仅对于棘皮动物群体内部的进化比较至关重要，而且对于更广泛的后口动物群体也很关键。在这里，我们结合免疫组织化学、原位杂交和纳米串nCounter技术来表征这种脆星的神经再生，以确定进化保守神经基因的时空表达。我们发现，对海胆和其他动物神经系统胚胎发育至关重要的关键基因，在成年脆星再生神经系统中也以层次化且时空受限的方式表达。