Xie Pengdong, Yang Yangyang, Gong Di, Yu Lirong, Han Ye, Zong Yuanyuan, Li Yongcai, Prusky Dov, Bi Yang
College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China.
Department of Food Science, Agricultural Research Organization, The Volcani Center, Rishon LeZion 7505101, Israel.
Antioxidants (Basel). 2022 Sep 10;11(9):1791. doi: 10.3390/antiox11091791.
Chitooligosaccharide (COS) is a degradation product of chitosan. Although COS increased fruit resistance by regulating the metabolism of reactive oxygen species (ROS), few reports are available on whether COS regulates ROS homeostasis at wounds of potato tubers during healing. In this study, COS increased gene expression and activities of NADPH oxidase and superoxide dismutase, and promoted the generation of O and HO. Moreover, COS increased gene expression and activities of catalase, peroxidase, and AsA-GSH cycle-related enzymes, as well as the levels of ascorbic acid and glutathione levels. In addition, COS elevated the scavenging ability of DPPH, ABTS, and FRAP, and reduced cell membrane permeability and malondialdehyde content. Taken together, COS could maintain cell membrane integrity by eliminating excessive HO and improving the antioxidant capacity in vitro, which contributes to the maintainance of cell membrane integrity at wounds of potato tubers during healing.
壳寡糖（COS）是壳聚糖的降解产物。尽管壳寡糖通过调节活性氧（ROS）代谢提高了果实抗性，但关于壳寡糖在马铃薯块茎伤口愈合过程中是否调节ROS稳态的报道较少。在本研究中，壳寡糖增加了NADPH氧化酶和超氧化物歧化酶的基因表达及活性，并促进了O和HO的产生。此外，壳寡糖增加了过氧化氢酶、过氧化物酶和AsA-GSH循环相关酶的基因表达及活性，以及抗坏血酸和谷胱甘肽水平。此外，壳寡糖提高了DPPH、ABTS和FRAP的清除能力，降低了细胞膜通透性和丙二醛含量。综上所述，壳寡糖可通过消除过量的HO和提高体外抗氧化能力来维持细胞膜完整性，这有助于在马铃薯块茎伤口愈合过程中维持细胞膜完整性。
