Li Yilin, Zhang Yalin, Su Xiaoming, Zou Pengfei, Wang Xinyang, Chen Jie, Zhu Xuan
Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Ornamental Aquarium Engineering Research Centre in University of Fujian Province, Fisheries College, Jimei University, Xiamen 361021, China.
School of Food Science and Bioengineering, Zhejiang Gongshang University, Hangzhou 310018, China.
Foods. 2022 Sep 18;11(18):2897. doi: 10.3390/foods11182897.
belongs to the Liedang family. Known as "desert ginseng", it has high medicinal value and plays important roles in endocrine regulation, neuroprotection, immune regulation, and other processes. Some studies have shown that single substances such as polysaccharides and phenylethanolside can affect intestinal microbiota, but few studies have studied the synergistic effect of various components in extracts on intestinal microbiota. Therefore, in this study, through an in vitro digestion model (Changdao Moni, CDMN) combined with 16S rRNA gene amplification sequencing technology and untargeted metabolomics technology, it was found that the two extracts all had significant effects on the enteric cavity and mucosal flora. Both extracts inhibited in the intestinal cavity and and in the intestinal mucosa and promoted and in the intestinal cavity and in the intestinal mucosa. The aqueous extract also inhibited . Both extracts also significantly increased the production of short-chain fatty acids, especially butyrate. The intake of extract had significant effects on the metabolic pathways related to amino acids and lipids. Indoles were upregulated by the aqueous extract but downregulated by the alcohol extract. In addition, the extract also had a significant effect on the hemolytic phosphorus esters. In conclusion, the two kinds of extracts have different effects on intestinal microbiota and its metabolism. This study provides guiding significance for the edibility and food development of .
属于藜科。被称为“沙漠人参”，具有很高的药用价值，在内分泌调节、神经保护、免疫调节等过程中发挥重要作用。一些研究表明，多糖和苯乙醇苷等单一物质可影响肠道微生物群，但很少有研究探讨提取物中各种成分对肠道微生物群的协同作用。因此，在本研究中，通过体外消化模型（Changdao Moni，CDMN）结合16S rRNA基因扩增测序技术和非靶向代谢组学技术，发现两种提取物对肠腔和黏膜菌群均有显著影响。两种提取物均抑制肠腔中的 以及肠黏膜中的 和 ，并促进肠腔中的 和 以及肠黏膜中的 。水提取物还抑制 。两种提取物还显著增加了短链脂肪酸的产生，尤其是丁酸盐。提取物的摄入对与氨基酸和脂质相关的代谢途径有显著影响。吲哚在水提取物中上调，但在醇提取物中下调。此外，提取物对溶血磷脂也有显著影响。总之，两种提取物对肠道微生物群及其代谢有不同影响。本研究为 的可食用性和食品开发提供了指导意义。
