Henderson G B, Fairlamb A H, Cerami A
Mol Biochem Parasitol. 1987 May;24(1):39-45. doi: 10.1016/0166-6851(87)90113-7.
A trypanothione-dependent peroxidase activity has been identified in the insect trypanosomatid Crithidia fasciculata and in the mammalian trypanosome Trypanosoma brucei. Using organic hydroperoxides as oxidant, specific peroxidase activities in these organisms are 5.0 and 1.0 nmol min-1 (10(8) cells)-1 respectively. The T. brucei peroxidase had an activity of 0.4 nmol min-1 (10(8) cells)-1 using hydrogen peroxide as oxidant. The enzyme is specific for the N1,N8-bis(glutathionyl)spermidine conjugate (dihydro-trypanothione); N1-mono-glutathionylspermidine is not a substrate. Experiments to demonstrate that this parasite peroxidase may contain selenium were inconclusive. However, bloodstream T. brucei can incorporate radiolabelled selenite into proteins.
在昆虫锥虫克氏锥虫和哺乳动物锥虫布氏锥虫中已鉴定出一种依赖于三硫代谷胱甘肽的过氧化物酶活性。以有机氢过氧化物作为氧化剂时，这些生物体中的特异性过氧化物酶活性分别为5.0和1.0 nmol min-1(10(8)个细胞)-1。以过氧化氢作为氧化剂时，布氏锥虫过氧化物酶的活性为0.4 nmol min-1(10(8)个细胞)-1。该酶对N1,N8-双(谷胱甘肽基)亚精胺共轭物(二氢三硫代谷胱甘肽)具有特异性；N1-单谷胱甘肽基亚精胺不是底物。证明这种寄生虫过氧化物酶可能含硒的实验尚无定论。然而，血液中的布氏锥虫可以将放射性标记的亚硒酸盐掺入蛋白质中。
