School of Chemistry and Chemical Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
Department of Biotechnology, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan.
Molecules. 2022 Sep 6;27(18):5754. doi: 10.3390/molecules27185754.
Protein corona composition and precise physiological understanding of differentially expressed proteins are key for identifying disease biomarkers. In this report, we presented a distinctive quantitative proteomics table of molecular cell signaling differentially expressed proteins of corona that formed on iron carbide nanoparticles (NPs). High-performance liquid chromatography/electrospray ionization coupled with ion trap mass analyzer (HPLC/ESI-Orbitrap) and MASCOT helped quantify 142 differentially expressed proteins. Among these proteins, 104 proteins showed upregulated behavior and 38 proteins were downregulated with respect to the control, whereas 48, 32 and 24 proteins were upregulated and 8, 9 and 21 were downregulated CW (control with unmodified NPs), CY (control with modified NPs) and WY (modified and unmodified NPs), respectively. These proteins were further categorized on behalf of their regularity, locality, molecular functionality and molecular masses using gene ontology (GO). A STRING analysis was used to target the specific range of proteins involved in metabolic pathways and molecular processing in different kinds of binding functionalities, such as RNA, DNA, ATP, ADP, GTP, GDP and calcium ion bindings. Thus, this study will help develop efficient protocols for the identification of latent biomarkers in early disease detection using protein fingerprints.
蛋白质冠的组成和差异表达蛋白的精确生理认识是识别疾病生物标志物的关键。在本报告中，我们展示了一个独特的定量蛋白质组学表，列出了碳化铁纳米颗粒（NPs）形成的蛋白质冠中差异表达的分子细胞信号蛋白。高效液相色谱/电喷雾电离耦合离子阱质量分析仪（HPLC/ESI-Orbitrap）和 Mascot 帮助定量了 142 个差异表达蛋白。在这些蛋白质中，与对照组相比，有 104 种蛋白质表现出上调行为，38 种蛋白质下调，而 CW（未修饰 NPs 的对照）、CY（修饰 NPs 的对照）和 WY（修饰和未修饰 NPs）分别有 48、32 和 24 种蛋白质上调，有 8、9 和 21 种蛋白质下调。这些蛋白质进一步根据其规律、位置、分子功能和分子质量使用基因本体论（GO）进行分类。STRING 分析用于针对涉及不同结合功能（如 RNA、DNA、ATP、ADP、GTP、GDP 和钙离子结合）的代谢途径和分子处理的特定范围内的蛋白质。因此，这项研究将有助于开发使用蛋白质指纹识别早期疾病检测中潜在生物标志物的有效方案。
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