Library, University of South China, Hengyang 421001, China.
School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China.
Sensors (Basel). 2022 Sep 15;22(18):6987. doi: 10.3390/s22186987.
Developing a fluorescent probe for UO, which is resistant to interference from other ions such as Cu and can be applied in acidic and high-water systems, has been a major challenge. In this study, a "turn-off" fluorescent probe for triamine-modified flavonoid derivatives, 2-triphenylamine-3-hydroxy-4H-chromen-4-one (abbreviated to HTPAF), was synthesized. In the solvent system of dimethyl sulfoxide:HO (abbreviated to DMSO:HO) (/ = 5:95 pH = 4.5), the HTPAF solution was excited with 364 nm light and showed a strong fluorescence emission peak at 474 nm with a Stokes shift of 110 nm. After the addition of UO, the fluorescence at 474 nm was quenched. More importantly, there was no interference in the presence of metal ions (Pb, Cd, Cr, Fe, Co, Th, La, etc.), especially Cu and Al. It is worth noting that the theoretical model for the binding of UO to HTPAF was derived by more detailed density functional theory (DFT) calculations in this study, while the coordination mode was further verified using HRMS, FT-IR and HNMR, demonstrating a coordination ratio of 1:2. In addition, the corresponding photo-induced electron transfer (PET) fluorescence quenching mechanism was also proposed.
开发一种对 UO 具有抗干扰性的荧光探针，这种探针能够抵抗 Cu 等其他离子的干扰，并且可以应用于酸性和高水体系，这一直是一个重大的挑战。在这项研究中，我们合成了一种三胺修饰黄酮衍生物的“关闭”型荧光探针，即 2-三苯胺-3-羟基-4H-色烯-4-酮（缩写为 HTPAF）。在二甲亚砜：HO（缩写为 DMSO:HO）溶剂体系中（/ = 5:95，pH = 4.5），HTPAF 溶液在 364nm 光激发下显示出一个在 474nm 处具有强荧光发射峰的 Stokes 位移为 110nm 的荧光。加入 UO 后，在 474nm 处的荧光被猝灭。更重要的是，即使存在金属离子（Pb、Cd、Cr、Fe、Co、Th、La 等），特别是 Cu 和 Al，也没有干扰。值得注意的是，本研究通过更详细的密度泛函理论（DFT）计算推导出了 UO 与 HTPAF 结合的理论模型，而配位模式则通过高分辨率质谱（HRMS）、傅里叶变换红外光谱（FT-IR）和核磁共振（HNMR）进一步得到验证，证明了配位比为 1:2。此外，还提出了相应的光诱导电子转移（PET）荧光猝灭机制。
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