Wang Dongyuan, Yin Qiang, Zheng Ming, Xie Yongbo, He Wen, Li Zheng, Hou Sanying, Wang Hongqing
Hunan Key Laboratory for The Design and Application of Actinide Complexes, University of South China, Hengyang, Hunan 421001, PR China.
School of Chemistry and Chemical Engineering, University of South China, Hengyang, Hunan 421001, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Apr 15;251:119480. doi: 10.1016/j.saa.2021.119480. Epub 2021 Jan 19.
It is of great importance to design a fluorescent sensor with high selectivity, sensitivity and large Stokes shift to zinc detection for environmental water sample and in vivo. Herein, A novel Zn fluorescent sensor with larger Stokes shift (110 nm) 1-((5-(4-(diphenylamino)phenyl)pyridine-2-imino)methyl)naphthalene-2-ol (abbr. TPA-PN) was designed and synthesized. In DMF-HO (V: V = 1: 1, pH = 7.0) solution, it could achieve high selectivity and sensitivity to Zn, there was a linear responsive range of 0-20 μM of concentration of Zn ions for the sensor, the detection limit was as low as 19.134 nM and the binding constant was calculated to be 3.24 × 10 M. The species of TPA-PN and zinc were clarified at different pH. Besides, the interaction properties and fluorescence mechanism were demonstrated by the species theory, density functional theory (DFT) calculation, H NMR titration, FT-IR and MS. Most importantly, it provided a new real-time, on-site method and showed excellent potential in-vivo imaging ability.
设计一种对环境水样和体内锌检测具有高选择性、高灵敏度和大斯托克斯位移的荧光传感器具有极其重要的意义。在此，设计并合成了一种具有较大斯托克斯位移（110 nm）的新型锌荧光传感器1-((5-(4-(二苯胺基)苯基)吡啶-2-亚氨基)甲基)萘-2-醇（简称TPA-PN）。在DMF-H₂O（V:V = 1:1，pH = 7.0）溶液中，它对锌具有高选择性和高灵敏度，该传感器对锌离子浓度的线性响应范围为0-20 μM，检测限低至19.134 nM，结合常数经计算为3.24×10⁶ M⁻¹。在不同pH值下明确了TPA-PN与锌的物种情况。此外，通过物种理论、密度泛函理论（DFT）计算、¹H NMR滴定、FT-IR和MS对相互作用性质和荧光机理进行了论证。最重要的是，它提供了一种新的实时、现场方法，并在体内成像能力方面显示出优异的潜力。
