Zhai Lu, Xie Lingli, Xu JinSong, Xu Benbo, Dong Jing, Zhang XueKun
Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, Hubei Key Laboratory of Waterlogging Disaster and Agricultural Use of Wetland, Yangtze University, Hubei, China.
College of Life Science, Yangtze University, Hubei, China.
Front Plant Sci. 2022 Sep 6;13:998698. doi: 10.3389/fpls.2022.998698. eCollection 2022.
Thidiazuron (TDZ) is a novel and efficient cytokinin commonly used in tissue culture, and numerous studies have demonstrated that TDZ can increase berry size. However, no study to date has explored the effect of TDZ on seed size of and the mechanism. To shed light on the effect of TDZ on the seed size of , four different concentrations of TDZ were applied to . Results indicated that TDZ treatment could increase the seed diameter and silique length of to varying degrees and 100 and 200 μmol/L TDZ treatments were the most effective with a 3.6 and 4.6% increase in seed diameter, respectively. In addition, the yield of was also substantially increased under TDZ treatment. On the other hand, confocal micrographs of embryos and cotyledon cells suggested that embryos and their cotyledon epidermal cells treated with 200 μmol/L TDZ were obviously larger in size than the control. Furthermore, TDZ promoted the upregulation of some key maternal tissue growth-related genes, including two G-protein signaling genes ( and ) and two transcriptional regulators ( and ). The expression analysis of genes related to the auxin metabolic pathways, G-protein signaling, endosperm growth and transcriptional regulators confirmed that treatment with TDZ negatively regulated the key genes , , , , and during bud development stage and florescence. The results strongly suggested that TDZ might regulate the transcriptional levels of key genes involved in auxin metabolic pathways, G-protein signaling, endosperm growth and transcriptional regulators, which resulted in bigger cotyledon epidermal cells and seed size in . This study explored the mechanism of TDZ treatment on the seed size of and provided an important reference for improving rapeseed yield.
噻苯隆（TDZ）是一种常用于组织培养的新型高效细胞分裂素，众多研究表明TDZ可增大浆果大小。然而，迄今为止尚无研究探讨TDZ对[植物名称]种子大小的影响及其机制。为阐明TDZ对[植物名称]种子大小的影响，对[植物名称]施加了四种不同浓度的TDZ。结果表明，TDZ处理能不同程度地增加[植物名称]的种子直径和角果长度，其中100和200μmol/L TDZ处理效果最佳，种子直径分别增加了3.6%和4.6%。此外，TDZ处理下[植物名称]的产量也显著提高。另一方面，胚胎和子叶细胞的共聚焦显微镜图像显示，用200μmol/L TDZ处理的胚胎及其子叶表皮细胞明显比对照大。此外，TDZ促进了一些与母体组织生长相关的关键基因的上调，包括两个G蛋白信号基因（[基因名称1]和[基因名称2]）和两个转录调节因子（[基因名称3]和[基因名称4]）。对生长素代谢途径、G蛋白信号、胚乳生长和转录调节因子相关基因的表达分析证实，在芽发育阶段和花期，TDZ处理对关键基因[基因名称5]、[基因名称6]、[基因名称7]、[基因名称8]和[基因名称9]具有负调控作用。结果强烈表明，TDZ可能调节生长素代谢途径、G蛋白信号、胚乳生长和转录调节因子中关键基因的转录水平，从而导致[植物名称]子叶表皮细胞和种子变大。本研究探讨了TDZ处理对[植物名称]种子大小的影响机制，为提高油菜产量提供了重要参考。
