Zhang Fan, Mao Changqing, Cao Siyu, Zhang Runchi, Guo Yi, Chen Guifang, Feng Chang
Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai, China.
Front Chem. 2022 Sep 6;10:1005315. doi: 10.3389/fchem.2022.1005315. eCollection 2022.
Bio-nanomicelles based on biomaterials such as nucleic acids, peptides, glycans, and lipids have developed rapidly in the field of bioanalysis. Although DNA and peptides have unique advantages, unfortunately, there are few bio-nanomicelles integrating DNA with peptides. Here, we designed a peptide-DNA hybrid bio-nanomicelle for the activity detection of caspase-3. The detection mechanism is based on caspase-3 specific recognition and cleavage of peptide substrates, which owns high sensitivity and selectivity. Under optimal conditions, the detection of caspase-3 activity can be achieved using our designed bio-nanomicelles and the detection limit is 0.72 nM. Furthermore, the proposed method was also successfully applied for the detection of caspase-3 in cell lysate samples after apoptosis-inducing.
基于核酸、肽、聚糖和脂质等生物材料的生物纳米胶束在生物分析领域发展迅速。尽管DNA和肽具有独特优势，但遗憾的是，将DNA与肽整合的生物纳米胶束很少。在此，我们设计了一种用于检测caspase-3活性的肽-DNA杂化生物纳米胶束。检测机制基于caspase-3对肽底物的特异性识别和切割，具有高灵敏度和选择性。在最佳条件下，使用我们设计的生物纳米胶束可实现对caspase-3活性的检测，检测限为0.72 nM。此外，所提出的方法还成功应用于诱导凋亡后细胞裂解液样品中caspase-3的检测。
