MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
Management Office of Dafeng Milu National Nature Reserve, Yancheng, 224136, China.
Food Funct. 2022 Oct 17;13(20):10501-10515. doi: 10.1039/d2fo02125k.
In this study, a novel heteropolysaccharide (EPS 7-4) with a molecular weight of 53 387 Da was isolated from , and it was mainly composed of mannose (36.9%) and glucose (30.8%). EPS 7-4 showed excellent inhibitory effects on the proliferation, biofilm formation, and virulence factor gene expression of () by disrupting the integrity of the bacterial wall. Furthermore, EPS 7-4 can effectively restrict bacterial translocation, upregulate the abundance of spp. and spp., and alleviate the induced severe inflammatory response in the intestinal tract of mice. Besides, we demonstrated that EPS 7-4 can protect mice by inhibiting induced pyroptosis, with the mechanism that EPS 7-4 affects ASC oligomerization during inflammasome-mediated pyroptosis. Therefore, due to its excellent anti-bacterial and anti-inflammatory abilities, EPS 7-4 is a promising health regulator owing to its excellent antibacterial and anti-inflammatory abilities.
在这项研究中，从 中分离得到了一种分子量为 53387 Da 的新型杂多糖（EPS7-4），它主要由甘露糖（36.9%）和葡萄糖（30.8%）组成。EPS7-4 通过破坏细菌壁的完整性，对 （）的增殖、生物膜形成和毒力因子基因表达表现出优异的抑制作用。此外，EPS7-4 可以有效地限制细菌易位，上调 spp. 和 spp. 的丰度，并减轻 在小鼠肠道中引起的严重炎症反应。此外，我们证明 EPS7-4 可以通过抑制 诱导的细胞焦亡来保护小鼠，其机制是 EPS7-4 在炎症小体介导热激性细胞焦亡过程中影响 ASC 寡聚化。因此，由于其优异的抗菌和抗炎能力，EPS7-4 有望成为一种有前途的健康调节剂。
