Department of Otolaryngology and Head and Neck Surgery, Faculty of Medicine, University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.
Department of Otolaryngology and Head and Neck Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
Sci Rep. 2022 Sep 23;12(1):15911. doi: 10.1038/s41598-022-19842-w.
We investigated whether the oxidoreductase cofactor pyrroloquinoline quinone (PQQ) prevents noise-induced and age-related hearing loss (NIHL and ARHL) in mice. To assess NIHL, 8 week-old mice with and without PQQ administration were exposed to noise for 4 h. PQQ was orally administered for one week before and after noise exposure and subcutaneously once before noise exposure. For ARHL evaluation, mice were given drinking water with or without PQQ starting at 2 months of age. In the NIHL model, PQQ-treated mice had auditory brainstem response (ABR) thresholds of significantly reduced elevation at 8 kHz, a significantly increased number of hair cells at the basal turn, and significantly better maintained synapses beneath the inner hair cells compared to controls. In the ARHL model, PQQ significantly attenuated the age-related increase in ABR thresholds at 8 and 32 kHz at 10 months of age compared to controls. In addition, the hair cells, spiral ganglion cells, ribbon synapses, stria vascularis and nerve fibers were all significantly better maintained in PQQ-treated animals compared to controls at 10 months of age. These physiological and histological results demonstrate that PQQ protects the auditory system from NIHL and ARHL in mice.
我们研究了氧化还原酶辅因子吡咯并喹啉醌（PQQ）是否可以预防小鼠的噪声诱导性听力损失（NIHL）和年龄相关性听力损失（ARHL）。为了评估 NIHL，我们给有和没有 PQQ 给药的 8 周大的小鼠暴露在噪声中 4 小时。PQQ 在噪声暴露前后口服给药一周，并在噪声暴露前皮下给药一次。为了评估 ARHL，从 2 个月大开始，小鼠饮用含有或不含有 PQQ 的饮用水。在 NIHL 模型中，与对照组相比，PQQ 处理的小鼠在 8 kHz 时的听性脑干反应（ABR）阈值升高显著降低，基底回的毛细胞数量显著增加，以及内毛细胞下的突触保持得更好。在 ARHL 模型中，与对照组相比，10 个月大时，PQQ 显著减弱了 ABR 阈值在 8 和 32 kHz 处与年龄相关的增加。此外，与对照组相比，在 10 个月大时，PQQ 处理的动物的毛细胞、螺旋神经节细胞、带状突触、血管纹和神经纤维都得到了更好的维持。这些生理和组织学结果表明，PQQ 可保护小鼠的听觉系统免受 NIHL 和 ARHL 的侵害。
