Metabolomics Laboratory, Clinical Research Centre, Medical University of Bialystok, Bialystok, Poland.
Department of Ophthalmology, Medical University of Bialystok, Bialystok, Poland.
Methods Mol Biol. 2023;2571:149-155. doi: 10.1007/978-1-0716-2699-3_15.
Aqueous humor (AH) is a transparent fluid that fills the anterior segment of the eye. The composition and level of metabolites in AH are important for understanding its physiology and changes caused by the occurrence of eye disease. A simple method for the preparation and analysis of AH samples was developed using the liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) technique. The analyses were performed using two types of chromatography: reversed-phase liquid chromatography-mass spectrometry (LC-RP-MS) and hydrophilic interaction liquid chromatography-mass spectrometry (LC-HILIC-MS), in the sample prepared by one protocol.
房水是充满眼球前段的透明液体。房水中代谢产物的组成和水平对于了解其生理学以及眼部疾病发生引起的变化非常重要。本研究使用液相色谱-四级杆飞行时间质谱（LC-QTOF-MS）技术开发了一种简单的房水样品制备和分析方法。该方法通过一种方案制备样品，使用两种类型的色谱：反相液相色谱-质谱（LC-RP-MS）和亲水相互作用液相色谱-质谱（LC-HILIC-MS）进行分析。
