Arapitsas Panagiotis, Mattivi Fulvio
Department of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach (FEM), San Michele all'Adige, Italy.
Center Agriculture Food Environment, University of Trento, San Michele all'Adige, Italy.
Methods Mol Biol. 2018;1738:225-235. doi: 10.1007/978-1-4939-7643-0_16.
This chapter describes a protocol for the analysis of the metabolomic fingerprint of wine by liquid chromatography-mass spectrometry. The straightforward, optimized sample preparation procedure is limited to a single-step dilution with water or acetonitrile. The separation of wine analytes is carried out by two columns with orthogonal selectivity, including both reversed-phase (C18) and hydrophilic interaction (HILIC) chromatography, while the detection is assured by a high-resolution quadrupole time-of-flight mass spectrometer operating in negative and positive electrospray ionization mode, in order to obtain four different chromatograms for each sample. This validated protocol, or parts of it, could be applied in several oenological topic experimental designs, including wine quality and wine authenticity.
本章介绍了一种通过液相色谱-质谱联用分析葡萄酒代谢组指纹图谱的方法。简单、优化的样品制备程序仅限于用水或乙腈进行单步稀释。葡萄酒分析物的分离通过两根具有正交选择性的色谱柱进行，包括反相（C18）和亲水作用（HILIC）色谱，而检测则由一台在负离子和正离子电喷雾电离模式下运行的高分辨率四极杆飞行时间质谱仪进行，以便为每个样品获得四种不同的色谱图。这个经过验证的方法或其中的部分内容可应用于多个葡萄酒酿造学主题的实验设计，包括葡萄酒质量和葡萄酒真伪鉴定。
