Clinical Lipidomics Unit, Institute of Physiological Chemistry, University Medical Center of the Johannes Gutenberg University of Mainz, Mainz, Germany.
Institute of Physiological Chemistry, University Medical Center of the Johannes Gutenberg University of Mainz, Mainz, Germany.
Methods Mol Biol. 2023;2576:9-19. doi: 10.1007/978-1-0716-2728-0_2.
Extraction and quantification of endocannabinoids from biological tissues is essential to unravel their changes under physiological and pathophysiological conditions. We describe here an analytical protocol for the extraction of endocannabinoids, anandamide (archidonoyl ethanolamide, AEA) and 2-arachidonoyl glycerol (2-AG), and endocannabinoid-like lipids such as palmitoyl ethanolamide (PEA) and oleoyl ethanolamide (OEA), as well as arachidonic acid (AA) from biological tissues using liquid-liquid extraction method and simultaneous quantification by liquid chromatography multiple reaction monitoring (LC/MRM).
从生物组织中提取和定量内源性大麻素对于揭示它们在生理和病理生理条件下的变化至关重要。我们在这里描述了一种用于提取内源性大麻素、花生四烯酰乙醇胺（arachidonoyl ethanolamide，AEA）和 2-花生四烯酰甘油（2-arachidonoyl glycerol，2-AG）以及内源性大麻素样脂质，如棕榈酰乙醇酰胺（palmitoyl ethanolamide，PEA）和油酰乙醇酰胺（oleoyl ethanolamide，OEA）以及花生四烯酸（arachidonic acid，AA）的分析方案，该方案使用液-液萃取方法，并通过液相色谱多重反应监测（LC/MRM）进行同时定量。
