生物组织中内源性大麻素和类内源性大麻素脂质的提取与同步定量分析
Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-Like Lipids in Biological Tissues.
作者信息
Bindila Laura, Lutz Beat
机构信息
Institute for Physiological Chemistry, University Medical Center, Duesbergweg 6, 55128, Mainz, Germany.
出版信息
Methods Mol Biol. 2016;1412:9-18. doi: 10.1007/978-1-4939-3539-0_2.
Extraction and quantification of endocannabinoids (eCBs) from biological tissues are essential to unravel their changes in physiological and pathophysiological conditions. We describe here an analytical protocol for extraction of endocannabinoids, anandamide (AEA) and 2-arachidonoyl glycerol (2-AG), endocannabinoid-like lipids such as palmitoyl ethanolamide (PEA) and oleoyl ethanolamide (OEA), as well as arachidonic acid (AA) from biological tissues using liquid-liquid extraction method and simultaneous quantification by liquid chromatography multiple reaction monitoring (LC/MRM).
从生物组织中提取和定量内源性大麻素（eCBs）对于揭示其在生理和病理生理条件下的变化至关重要。我们在此描述一种分析方法，用于使用液-液萃取法从生物组织中提取内源性大麻素、花生四烯酸乙醇胺（AEA）和2-花生四烯酸甘油酯（2-AG）、内源性大麻素样脂质如棕榈酰乙醇胺（PEA）和油酰乙醇胺（OEA）以及花生四烯酸（AA），并通过液相色谱多反应监测（LC/MRM）进行同步定量。
Zotero 插件