Scion Crown Research Institute, Rotorua, New Zealand.
Methods Mol Biol. 2023;2566:243-259. doi: 10.1007/978-1-0716-2675-7_20.
Autofluorescence of plant tissues can be used as a label-free method to detect a range of phenolic-based cell wall components including lignin, suberin, and ferulate using widefield or confocal fluorescence microscopy. Likewise, fluorescently labeled antibodies can be used to localize specific carbohydrate molecules including arabinoxylan, β-1,4 galactan, glucomannan, glucuronoxylan, pectins, and xyloglucan. When combined, these two methods allow detailed study of topochemistry in different plant tissues for phenotyping of mutant varieties and plant biology studies. This article describes the protocols for fluorescent detection and imaging of molecules in plant cell walls using autofluorescence and immunofluorescence.
植物组织的自发荧光可以作为一种无标记的方法，使用宽场或共聚焦荧光显微镜来检测包括木质素、角质素和阿魏酸在内的一系列基于酚类的细胞壁成分。同样，荧光标记的抗体可以用于定位特定的碳水化合物分子，包括阿拉伯木聚糖、β-1,4 半乳糖、葡甘露聚糖、葡萄糖醛酸木聚糖、果胶和木葡聚糖。当这两种方法结合使用时，可以对不同植物组织的拓扑化学进行详细研究，用于突变体品种的表型分析和植物生物学研究。本文描述了使用自发荧光和免疫荧光检测和成像植物细胞壁中分子的方案。
