Department of Plant Science and ECP3-Multi-Scale Imaging Facility, McGill University, Sainte-Anne-de-Bellevue, QC, Canada.
Methods Mol Biol. 2023;2566:269-279. doi: 10.1007/978-1-0716-2675-7_22.
The plant cell wall comprises various types of macromolecules whose abundance and spatial distribution change dynamically and are crucial for plant architecture. High-resolution live cell imaging of plant cell wall components is, therefore, a powerful tool for plant cell biology and plant developmental biology. To acquire suitable data, the experimental setup for staining and imaging of non-fixed samples must be straightforward and avoid creating stress-induced artifacts. We present a detailed sample preparation and live image acquisition protocol for fluorescence visualization of cell wall components using commercially available probes and stains.
植物细胞壁由多种类型的大分子组成，其丰度和空间分布动态变化，对植物结构至关重要。因此，对植物细胞壁成分进行高分辨率活细胞成像分析是植物细胞生物学和植物发育生物学的有力工具。为了获取合适的数据，非固定样品的染色和成像实验方案必须简单直接，避免产生应激诱导的假象。我们提供了一个详细的样品制备和活细胞成像采集方案，使用市售探针和染料对细胞壁成分进行荧光可视化。
