Department of Plant Science, McGill University, Macdonald Campus, Ste-Anne-de-Bellevue, Québec, Canada.
J Microsc. 2020 Jun;278(3):164-181. doi: 10.1111/jmi.12895. Epub 2020 May 14.
Plant cell walls constitute the extracellular matrix surrounding plant cells and are composed mainly of polysaccharides. The chemical makeup of the primary plant cell wall, and specifically, the abundance, localization and arrangement of the constituting polysaccharides are intimately linked with growth, morphogenesis and differentiation in plant cells. Visualization of the cell wall components is, therefore, a crucial tool in plant cell developmental studies. In this technical update, we present protocols for fluorescence visualization of cellulose and pectin in selected plant tissues and illustrate examples of some of the available labels that hold promise for live imaging of plant cell wall expansion and morphogenesis.
植物细胞壁构成了植物细胞周围的细胞外基质，主要由多糖组成。初生植物细胞壁的化学成分，特别是构成多糖的丰度、定位和排列，与植物细胞的生长、形态发生和分化密切相关。因此，细胞壁成分的可视化是植物细胞发育研究的重要工具。在本技术更新中，我们提供了在选定植物组织中荧光可视化纤维素和果胶的方案，并举例说明了一些有前途的标签，这些标签可用于植物细胞壁扩展和形态发生的活体成像。
