Department of Metrology and Optoelectronics, Faculty of Electronics, Telecommunications and Informatics, Gdańsk University of Technology, Gdańsk, Poland.
Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdańsk, Gdańsk, Poland.
J Biophotonics. 2023 Jan;16(1):e202200186. doi: 10.1002/jbio.202200186. Epub 2022 Oct 5.
In this paper, we present an investigation of the influence of the temperature on the sensing of biological samples. We used biofunctionalized microsphere-based fiber-optic sensor to detect immunoglobulin G attached to the sensor head at temperatures relevant in biological research: 5°C, 25°C, and 55°C. The construction of the sensor allowed us to perform measurements in the small amount of solution. The results of our experiment confirm substantial changes in the measured reflected optical power, indicating the need to control the temperature during such measurements. The sensitivity of the sensor used in this research is 8.82 nW/°C. Coefficient R was also calculated and it equals 0.998, which shows good fit between theoretical linear fit and obtained measured data.
在本文中，我们研究了温度对生物样本传感的影响。我们使用基于生物功能化微球的光纤传感器来检测附着在传感器头部的免疫球蛋白 G，温度分别为 5°C、25°C 和 55°C，这些温度与生物研究相关。传感器的构建允许我们在少量溶液中进行测量。我们的实验结果证实了所测量的反射光功率发生了实质性变化，这表明在进行此类测量时需要控制温度。本研究中使用的传感器的灵敏度为 8.82nW/°C。还计算了相关系数 R，其等于 0.998，这表明理论线性拟合与获得的实测数据之间具有良好的拟合度。
